The Peculiar Case of the Hyper‐thermostable Pyrimidine Nucleoside Phosphorylase from Thermus thermophilus **
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DOI: 10.1002/cbic.202000679
Abstract: The poor solubility of many nucleosides and nucleobases in aqueous solution demands harsh reaction conditions (base, heat, cosolvent) in nucleoside phosphorylase‐catalyzed processes to facilitate substrate loading beyond the low millimolar range. This, in turn, requires… read more here.
Keywords: pyrimidine; thermus thermophilus; pyrimidine nucleoside; phosphorylase thermus ... See more keywords
A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides
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DOI: 10.1007/s10529-017-2423-1
Abstract: ObjectiveTo isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.ResultsBbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP… read more here.
Keywords: borstelensis lk01; brevibacillus borstelensis; pyrimidine nucleoside; bbpynp ... See more keywords
Interaction Of 5-Substituted Pyrimidine Nucleoside Analogues And M.Tuberculosis: A View Through An Electron Microscope.
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DOI: 10.1016/j.biochi.2020.03.004
Abstract: The data of transmission electron microscopy (TEM) on morphology of M. tuberculosis H37Rv bacterial cells treated with four analogues of pyrimidine nucleosides with different substituents at 5 position of base are presented. We showed that… read more here.
Keywords: interaction substituted; substituted pyrimidine; tuberculosis; pyrimidine nucleoside ... See more keywords
Synthesis of pyrimidine nucleoside and amino acid conjugates
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DOI: 10.1016/j.tetlet.2020.152598
Abstract: Abstract The synthesis of novel pyrimidine nucleoside bioconjugates with amino acids is presented. The N4-amino acid-acylated 2′-deoxycytidine analogues, modified with various amino acids, were synthesized using a three-step synthesis and obtained in moderate overall yields.… read more here.
Keywords: nucleoside amino; synthesis pyrimidine; amino acid; pyrimidine nucleoside ... See more keywords
pH-Independent Heat Capacity Changes during Phosphorolysis Catalyzed by the Pyrimidine Nucleoside Phosphorylase from Geobacillus thermoglucosidasius.
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DOI: 10.1021/acs.biochem.1c00156
Abstract: Enzyme-catalyzed reactions sometimes display curvature in their Eyring plots in the absence of denaturation, indicative of a change in activation heat capacity. However, the effects of pH and (de)protonation on this phenomenon have remained unexplored.… read more here.
Keywords: phosphorylase geobacillus; pyrimidine nucleoside; heat; heat capacity ... See more keywords