Computational and mutational hotspot analysis of mycobacterial inorganic pyrophosphatase and virtual screening of natural compounds to discover contemporary drug candidates: Trilateral approach that explores its GTPase activity.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.2381
Abstract: Mycobacterial inorganic pyrophosphatase (Mt-PPa) play essential role in bacterial in vitro and in vivo survival. This family of proteins reacts on inorganic pyrophosphate to release inorganic phosphate and protect bacteria from pyrophosphate toxicity. Rv3628 (Mt-PPa)… read more here.
Keywords: pyrophosphatase; virtual screening; inorganic pyrophosphatase; ppa ... See more keywords
The Asp1 pyrophosphatase from S. pombe hosts a [2Fe-2S]2+ cluster in vivo
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Biological Inorganic Chemistry"
DOI: 10.1007/s00775-020-01840-w
Abstract: The Schizosaccharomyces pombe Asp1 protein is a bifunctional kinase/pyrophosphatase that belongs to the highly conserved eukaryotic diphosphoinositol pentakisphosphate kinase PPIP5K/Vip1 family. The N-terminal Asp1 kinase domain generates specific high-energy inositol pyrophosphate (IPP) molecules, which are… read more here.
Keywords: cluster vivo; pyrophosphatase; spectroscopy; cluster ... See more keywords
Characterization of an archaeal inorganic pyrophosphatase from Sulfolobus islandicus using a [31P]-NMR-based assay.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.11.019
Abstract: Inorganic pyrophosphatase catalyzes the conversion of pyrophosphate to phosphate and is often critical for driving reactions forward in cellular processes such as nucleic acid and protein synthesis. Commonly used methods for quantifying pyrophosphatase enzyme activity… read more here.
Keywords: 31p nmr; pyrophosphatase; sulfolobus islandicus; based assay ... See more keywords
Molecular cloning and functional characterisation of an H+-pyrophosphatase from Iris lactea
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18032-3
Abstract: Tonoplast H+-pyrophosphatases (VPs) mediate vacuolar Na+ sequestration, a process important for salt tolerance of plants. The function of VP in the highly drought- and salt-tolerant perennial Iris lactea under salt stress is unclear. Here, we… read more here.
Keywords: lactea; salt stress; pyrophosphatase; iris lactea ... See more keywords
Activities and Structure-Function Analysis of Fission Yeast Inositol Pyrophosphate (IPP) Kinase-Pyrophosphatase Asp1 and Its Impact on Regulation of pho1 Gene Expression
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.01034-22
Abstract: Expression of the fission yeast phosphate regulon is sensitive to the intracellular level of the inositol pyrophosphate (IPP) signaling molecule 1,5-IP8. IP8 dynamics are determined by Asp1, a bifunctional enzyme comprising N-terminal IPP 1-kinase and… read more here.
Keywords: pyrophosphatase; asp1; ip8; synthesis ... See more keywords
Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1159965
Abstract: Introduction: NADH pyrophosphatase, a hydrolase catalyzing the phosphate bond of NADH to reduced nicotinamide mononucleotide, has potential applications in the food, cosmetic and pharmaceutical industry. Methods: Here, we investigated the effects of vector screening, promoter… read more here.
Keywords: pyrophosphatase; reduced nicotinamide; protein engineering; production ... See more keywords
Catalytic Asymmetry in Homodimeric H+-Pumping Membrane Pyrophosphatase Demonstrated by Non-Hydrolyzable Pyrophosphate Analogs
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22189820
Abstract: Membrane-bound inorganic pyrophosphatase (mPPase) resembles the F-ATPase in catalyzing polyphosphate-energized H+ and Na+ transport across lipid membranes, but differs structurally and mechanistically. Homodimeric mPPase likely uses a “direct coupling” mechanism, in which the proton generated… read more here.
Keywords: pyrophosphatase; non hydrolyzable; catalytic asymmetry; active site ... See more keywords
Good-Practice Non-Radioactive Assays of Inorganic Pyrophosphatase Activities
Sign Up to like & getrecommendations! Published in 2021 at "Molecules"
DOI: 10.3390/molecules26082356
Abstract: Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that converts pyrophosphate (PPi) to phosphate and, in this way, controls numerous biosynthetic reactions that produce PPi as a byproduct. PPase activity is generally assayed by measuring the… read more here.
Keywords: pyrophosphatase; practice non; radioactive assays; inorganic pyrophosphatase ... See more keywords
Pyrophosphatase 1 expression is associated with future recurrence and overall survival in Chinese patients with intrahepatic cholangiocarcinoma.
Sign Up to like & getrecommendations! Published in 2018 at "Oncology letters"
DOI: 10.3892/ol.2018.8278
Abstract: The inorganic pyrophosphatase gene (PPA1) encodes inorganic pyrophosphatase, an enzyme that catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate, and has been revealed to be dysregulated in several types of human cancer. However, the role… read more here.
Keywords: pyrophosphatase; ppa1; intrahepatic cholangiocarcinoma; future recurrence ... See more keywords