In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase
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DOI: 10.1002/1873-3468.70075
Abstract: The dimeric glycyl radical enzyme pyruvate formate‐lyase (PflB; formate acetyltransferase 1) cleaves pyruvate with hypothetical half‐site reactivity to formate and acetyl‐CoA. The radical introduced onto residue G734 of PflB is transiently transferred to C419 of… read more here.
Keywords: formate lyase; variant; formate; glycyl radical ... See more keywords
A synthetic bacterial microcompartment as production platform for pyruvate from formate and acetate
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DOI: 10.1073/pnas.2201330119
Abstract: Bacteria do not have organelles. This textbook statement is as simple as it is false. An organelle is defined as a specialized structure inside a cell that has specific functions, such as the mitochondrium, the… read more here.
Keywords: synthetic bacterial; production platform; pyruvate formate; platform pyruvate ... See more keywords
Coordinated Expression of the Genes Encoding FocA and Pyruvate Formate-Lyase Is Important for Maintenance of Formate Homeostasis during Fermentative Growth of Escherichia coli
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DOI: 10.3390/fermentation9040382
Abstract: FocA is a pentameric membrane channel that translocates formic acid bidirectionally across the cytoplasmic membrane of Escherichia coli during fermentation. The focA gene is co-transcribed with pflB, which encodes pyruvate formate-lyase, the enzyme that generates… read more here.
Keywords: growth; escherichia coli; formate; formate lyase ... See more keywords