A Python script to design primers for overlap extension PCR to ligate two DNA fragments
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DOI: 10.7717/peerj.14283
Abstract: Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a… read more here.
Keywords: overlap extension; python script; dna fragments; design ... See more keywords