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   Articles with "qpcr" as a keyword



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PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

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recommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"

DOI: 10.1007/s00216-020-02490-2

Abstract: DNA analysis has seen an incredible development in terms of instrumentation, assays and applications over the last years. Massively parallel sequencing (MPS) and digital PCR are now broadly applied in research and diagnostics, and quantitative… read more here.

Keywords: qpcr; dna; analysis; inhibition ... See more keywords
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A newly developed BVDV-1 RT-qPCR Taqman assay based on Italian isolates: evaluation as a diagnostic tool

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recommendations! Published in 2017 at "Folia Microbiologica"

DOI: 10.1007/s12223-017-0497-8

Abstract: A single-step TaqMan® RT-qPCR was developed for the detection of bovine viral diarrhea virus type 1 (BVDV-1), an important pathogen of cattle worldwide. The assay was based on conserved 5’UTR sequences of Italian BVDV-1 isolates.… read more here.

Keywords: qpcr; taqman; assay based; bvdv ... See more keywords
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Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method

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recommendations! Published in 2017 at "Food and Environmental Virology"

DOI: 10.1007/s12560-017-9317-1

Abstract: Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV… read more here.

Keywords: environmental water; water samples; qpcr; isc qpcr ... See more keywords
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Development of a revised ICC-qPCR method used for Pseudorabies virus inactivation validation study of biologically sourced materials.

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recommendations! Published in 2020 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2020.113576

Abstract: To develop a precise and convenient method to evaluate the virus transmission risk of biologically sourced materials, an integrated cell culture-qPCR (ICC-qPCR) method for Pseudorabies virus (PRV) was established and revised for applications to this… read more here.

Keywords: biologically sourced; qpcr; icc qpcr; revised icc ... See more keywords
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Chronic Chagas disease: quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy.

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recommendations! Published in 2019 at "Acta tropica"

DOI: 10.1016/j.actatropica.2019.105167

Abstract: It is not currently known which individuals with chronic Chagas disease (ChD) will develop cardiopathy in a determined period and which will be maintained asymptomatic with normal routine laboratory tests all their lives. The parasite… read more here.

Keywords: qpcr; without cardiopathy; fed xenodiagnosis; chronic chagas ... See more keywords
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A comparison of two molecular methods for diagnosing leptospirosis from three different sample types in patients presenting with fever in Laos

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recommendations! Published in 2018 at "Clinical Microbiology and Infection"

DOI: 10.1016/j.cmi.2017.10.017

Abstract: Objectives To compare two molecular assays (rrs quantitative PCR (qPCR) versus a combined 16SrRNA and LipL32 qPCR) on different sample types for diagnosing leptospirosis in febrile patients presenting to Mahosot Hospital, Vientiane, Laos. Methods Serum,… read more here.

Keywords: two molecular; qpcr; 16srrna lipl32; lipl32 qpcr ... See more keywords
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A long-amplicon quantitative PCR assay with propidium monoazide to enumerate viable Listeria monocytogenes after heat and desiccation treatments.

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recommendations! Published in 2020 at "Food microbiology"

DOI: 10.1016/j.fm.2019.103310

Abstract: The objective of this study was to develop a qPCR method for specific enumeration of viable Listeria monocytogenes in food processing facilities and heat treated products. Primers specific for L. monocytogenes were designed to amplify… read more here.

Keywords: heat; qpcr; desiccation; qpcr method ... See more keywords
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Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.

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recommendations! Published in 2021 at "International journal of food microbiology"

DOI: 10.1016/j.ijfoodmicro.2021.109504

Abstract: Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting… read more here.

Keywords: lactic acid; css; cold smoked; acid bacteria ... See more keywords
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Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples

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recommendations! Published in 2021 at "International Journal of Infectious Diseases"

DOI: 10.1016/j.ijid.2021.07.054

Abstract: Objective: To validate and implement an optimised screening method for detection of SARS-CoV-2 RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR. Methods: 3-phased study conducted… read more here.

Keywords: qpcr; method; saliva samples; sars cov ... See more keywords
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Detection and quantification of the insect pest Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) in rice by qPCR

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recommendations! Published in 2017 at "Journal of Stored Products Research"

DOI: 10.1016/j.jspr.2017.02.002

Abstract: Abstract The early detection of insects during grain storage and processing remains a major issue for the cereal industry, especially when immature stages are hidden inside the grain kernels. For this reason, we developed a… read more here.

Keywords: detection quantification; detection; rhyzopertha dominica; qpcr ... See more keywords
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Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.

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recommendations! Published in 2018 at "Journal of virological methods"

DOI: 10.1016/j.jviromet.2017.11.012

Abstract: TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7… read more here.

Keywords: qpcr; phage; sybr green; green qpcr ... See more keywords