The challenge of standardizing CAR‐T cell monitoring: A comparison of two flow‐cytometry methods and correlation with qPCR technique
Sign Up to like & getrecommendations! Published in 2024 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24825
Abstract: Chimeric antigen receptor (CAR) T‐cell therapy is a breakthrough in hematologic malignancies, such as acute B lymphoblastic leukemia (B‐ALL). Monitoring this treatment is recommended, although standardized protocols have not been developed yet. This work compares… read more here.
Keywords: correlation; car cell; qpcr; car ... See more keywords
PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02490-2
Abstract: DNA analysis has seen an incredible development in terms of instrumentation, assays and applications over the last years. Massively parallel sequencing (MPS) and digital PCR are now broadly applied in research and diagnostics, and quantitative… read more here.
Keywords: qpcr; dna; analysis; inhibition ... See more keywords
Novel and simple qPCR system for the detection and quantification of the probiotic bacteria in food supplements
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Food Measurement and Characterization"
DOI: 10.1007/s11694-025-03181-1
Abstract: The food supplements gain an attractivity among consumers due to their pronounced health benefits. There is no legal frame for probiotic food supplements and the only controllable aspect is the agreement of the declaration with… read more here.
Keywords: detection quantification; food; qpcr; food supplements ... See more keywords
A newly developed BVDV-1 RT-qPCR Taqman assay based on Italian isolates: evaluation as a diagnostic tool
Sign Up to like & getrecommendations! Published in 2017 at "Folia Microbiologica"
DOI: 10.1007/s12223-017-0497-8
Abstract: A single-step TaqMan® RT-qPCR was developed for the detection of bovine viral diarrhea virus type 1 (BVDV-1), an important pathogen of cattle worldwide. The assay was based on conserved 5’UTR sequences of Italian BVDV-1 isolates.… read more here.
Keywords: qpcr; taqman; assay based; bvdv ... See more keywords
Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method
Sign Up to like & getrecommendations! Published in 2017 at "Food and Environmental Virology"
DOI: 10.1007/s12560-017-9317-1
Abstract: Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV… read more here.
Keywords: environmental water; water samples; qpcr; isc qpcr ... See more keywords
Development of a revised ICC-qPCR method used for Pseudorabies virus inactivation validation study of biologically sourced materials.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113576
Abstract: To develop a precise and convenient method to evaluate the virus transmission risk of biologically sourced materials, an integrated cell culture-qPCR (ICC-qPCR) method for Pseudorabies virus (PRV) was established and revised for applications to this… read more here.
Keywords: biologically sourced; qpcr; icc qpcr; revised icc ... See more keywords
Chronic Chagas disease: quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy.
Sign Up to like & getrecommendations! Published in 2019 at "Acta tropica"
DOI: 10.1016/j.actatropica.2019.105167
Abstract: It is not currently known which individuals with chronic Chagas disease (ChD) will develop cardiopathy in a determined period and which will be maintained asymptomatic with normal routine laboratory tests all their lives. The parasite… read more here.
Keywords: qpcr; without cardiopathy; fed xenodiagnosis; chronic chagas ... See more keywords
A comparison of two molecular methods for diagnosing leptospirosis from three different sample types in patients presenting with fever in Laos
Sign Up to like & getrecommendations! Published in 2018 at "Clinical Microbiology and Infection"
DOI: 10.1016/j.cmi.2017.10.017
Abstract: Objectives To compare two molecular assays (rrs quantitative PCR (qPCR) versus a combined 16SrRNA and LipL32 qPCR) on different sample types for diagnosing leptospirosis in febrile patients presenting to Mahosot Hospital, Vientiane, Laos. Methods Serum,… read more here.
Keywords: two molecular; qpcr; 16srrna lipl32; lipl32 qpcr ... See more keywords
A long-amplicon quantitative PCR assay with propidium monoazide to enumerate viable Listeria monocytogenes after heat and desiccation treatments.
Sign Up to like & getrecommendations! Published in 2020 at "Food microbiology"
DOI: 10.1016/j.fm.2019.103310
Abstract: The objective of this study was to develop a qPCR method for specific enumeration of viable Listeria monocytogenes in food processing facilities and heat treated products. Primers specific for L. monocytogenes were designed to amplify… read more here.
Keywords: heat; qpcr; desiccation; qpcr method ... See more keywords
Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.
Sign Up to like & getrecommendations! Published in 2021 at "International journal of food microbiology"
DOI: 10.1016/j.ijfoodmicro.2021.109504
Abstract: Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting… read more here.
Keywords: lactic acid; css; cold smoked; acid bacteria ... See more keywords
Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Infectious Diseases"
DOI: 10.1016/j.ijid.2021.07.054
Abstract: Objective: To validate and implement an optimised screening method for detection of SARS-CoV-2 RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR. Methods: 3-phased study conducted… read more here.
Keywords: qpcr; method; saliva samples; sars cov ... See more keywords