Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses
Sign Up to like & getrecommendations! Published in 2022 at "Archives of Virology"
DOI: 10.1007/s00705-022-05383-0
Abstract: Coronavirus disease 2019 (COVID-19) is an acute respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Other coronaviruses (CoVs) can also infect humans, although the majority cause only mild respiratory symptoms. Because early… read more here.
Keywords: sars cov; respiratory syndrome; qpcr assay; acute respiratory ... See more keywords
A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses.
Sign Up to like & getrecommendations! Published in 2021 at "Virologica Sinica"
DOI: 10.1007/s12250-021-00354-2
Abstract: Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging… read more here.
Keywords: detection; detection nine; human herpesviruses; qpcr assay ... See more keywords
A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections
Sign Up to like & getrecommendations! Published in 2020 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2020.102757
Abstract: Background Many health facilities in malaria endemic countries are dependent on Rapid diagnostic tests (RDTs) for diagnosis and some National Health Service (NHS) hospitals without expert microscopists rely on them for diagnosis out of hours.… read more here.
Keywords: protein; pfhrp2 pfhrp3; pfhrp3 deletions; qpcr assay ... See more keywords
Corrigendum to ‘A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections’
Sign Up to like & getrecommendations! Published in 2021 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2021.103261
Abstract: a Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, United Kingdom b PHE Malaria Reference Laboratory, London School of Hygiene & Tropical Medicine, United Kingdom c Centre of Statistics and Applications of… read more here.
Keywords: medicine; united kingdom; corrigendum novel; multiplex qpcr ... See more keywords
Rapid differential detection of Genotype I and III Japanese Encephalitis Virus from clinical samples by a novel duplex TaqMan probe-based RT-qPCR assay.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2020.113841
Abstract: Japanese Encephalitis (JE) is an acute infectious disease that threatens both human and pig populations throughout Asia. JE is caused by the Japanese Encephalitis Virus (JEV), of which Genotype III (GIII) had been the most… read more here.
Keywords: jev; taqman; japanese encephalitis; duplex taqman ... See more keywords
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2021.114333
Abstract: The increasing prevalence of N501Y variants of SARS-CoV-2 has kindled global concern due to their enhanced transmissibility. Genome sequencing is the gold standard method to identify the emerging variants of concern. But it is time-consuming… read more here.
Keywords: n501y variants; qpcr assay; sars cov; taqman minor ... See more keywords
Development and field evaluation of a species-specific mt-COI targeted SYBR-Green Real Time PCR for detection and quantification of Haemonchus contortus in cattle in Turkey.
Sign Up to like & getrecommendations! Published in 2019 at "Veterinary parasitology"
DOI: 10.1016/j.vetpar.2019.109020
Abstract: Haemonchus contortus is one of the most important gastrointestinal nematodes (GINs) infecting sheep, goats, and cattle worldwide. We developed a SYBR Green real-time PCR (qPCR) assay for detection and quantification of H. contortus by using… read more here.
Keywords: detection; coi; sybr green; qpcr assay ... See more keywords
Multiplex TaqMan qPCR Assay for Detection, Identification, and Quantification of Three Sclerotinia Species
Sign Up to like & getrecommendations! Published in 2022 at "Mycobiology"
DOI: 10.1080/12298093.2022.2131999
Abstract: Abstract White mold (or Sclerotinia stem rot), caused by Sclerotinia species, is a major air, soil, or seed-transmitted disease affecting numerous crops and wild plants. Microscopic or culture-based methods currently available for their detection and… read more here.
Keywords: sclerotinia; qpcr assay; detection identification; sclerotinia species ... See more keywords
A qPCR Assay for the Detection of Phytophthora cinnamomi Including an mRNA Protocol Designed to Establish Propagule Viability in Environmental Samples.
Sign Up to like & getrecommendations! Published in 2019 at "Plant disease"
DOI: 10.1094/pdis-09-18-1641-re
Abstract: Phytophthora cinnamomi causes root and collar rot in many plant species in natural ecosystems and horticulture. A species-specific primer and probe PCIN5 were designed based on a mitochondrial locus encoding subunit 2 of cytochrome c… read more here.
Keywords: qpcr assay; environmental samples; assay detection; protocol ... See more keywords
High Levels of Detection of Nonpneumococcal Species of Streptococcus in Saliva from Adults in the United States
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.05207-22
Abstract: Testing saliva samples with quantitative PCR (qPCR) improves the sensitivity of detection of pneumococcal carriage. The qPCR assay targeting lytA, the gene encoding the major pneumococcal autolysin, has become widely accepted for the identification of… read more here.
Keywords: pneumococcal carriage; saliva samples; detection; lyta ... See more keywords
Specific detection of Treponema pallidum in clinical samples: validation of a qPCR assay combining two genomic targets
Sign Up to like & getrecommendations! Published in 2022 at "Sexually Transmitted Infections"
DOI: 10.1136/sextrans-2021-055364
Abstract: Objectives We evaluated a real-time quantitative PCR (qPCR) for detection of the Treponema pallidum (TP) genome in clinical samples through simultaneous detection of two genomic targets. Methods We performed qPCR with TaqMan technology using two… read more here.
Keywords: detection treponema; detection; two genomic; qpcr assay ... See more keywords