In silico evaluation of the impact of Omicron variant of concern sublineage BA.4 and BA.5 on the sensitivity of RT‐qPCR assays for SARS‐CoV‐2 detection using whole genome sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28241
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) variant of concern (VoC) Omicron (B.1.1.529) has rapidly spread around the world, presenting a new threat to global public human health. Due to the large number of mutations… read more here.
Keywords: omicron; genome; variant concern; sensitivity qpcr ... See more keywords
Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2021.114288
Abstract: Previously, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), possibly representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (targeting genome segment 2)… read more here.
Keywords: bluetongue virus; novel bluetongue; btv; qpcr assays ... See more keywords
A novel quantitative PCR assay for testing bacteria directly from plate cultures using SYBR Green technology.
Sign Up to like & getrecommendations! Published in 2017 at "Pathology"
DOI: 10.1016/j.pathol.2017.06.004
Abstract: CI, confidence interval; CV, coefficient of variation; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation. a Number of batches of 30 tests analysed for mecA gene using standard suspensions of methicillin resistant Staphylococcus aureus… read more here.
Keywords: detection; plate cultures; pcr; sybr green ... See more keywords
A microfluidic platform for the simultaneous quantification of methanogen populations in anaerobic digestion processes
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Microbiology"
DOI: 10.1111/1462-2920.14589
Abstract: Methanogens are a diverse group of archaea that play a critical role in the global carbon cycle. The lack of appropriate molecular tools to simultaneously quantify numerous methanogenic taxa, however, has largely limited our ability… read more here.
Keywords: microfluidic platform; platform simultaneous; platform; qpcr assays ... See more keywords
RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2021.672562
Abstract: Background Several variants of the SARS-CoV-2 have been documented globally during the current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K SARS-CoV-2 mutations have been documented among the most relevant due to their potential pathogenic… read more here.
Keywords: n501y 70del; cov mutations; 70del k417n; qpcr assays ... See more keywords
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
Sign Up to like & getrecommendations! Published in 2021 at "Genes"
DOI: 10.3390/genes12040565
Abstract: For 1 year now, the world is undergoing a coronavirus disease-2019 (COVID-19) pandemic due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The most widely used method for COVID-19 diagnosis is the detection of… read more here.
Keywords: detection; new variants; evaluation; qpcr assays ... See more keywords
Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion
Sign Up to like & getrecommendations! Published in 2023 at "Pathogens"
DOI: 10.3390/pathogens12020290
Abstract: Domestic ruminant abortions due to infectious agents represent an important cause of economic losses in the agricultural industry. This study aimed to optimise and apply qPCR assays for detection of Chlamydiales in domestic ruminant abortion… read more here.
Keywords: ruminant abortion; domestic ruminant; detection; qpcr assays ... See more keywords
Sequencing a Strawberry Germplasm Collection Reveals New Viral Genetic Diversity and the Basis for New RT-qPCR Assays
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13081442
Abstract: Viruses are considered of major importance in strawberry (Fragaria × ananassa Duchesne) production given their negative impact on plant vigor and growth. Strawberry accessions from the National Clonal Germplasm Repository were screened for viruses using… read more here.
Keywords: genetic diversity; virus; qpcr assays; strawberry ... See more keywords