A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-40018-6
Abstract: N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches have been developed in order to… read more here.
Keywords: methylation; qpcr based; rna; relative quantification ... See more keywords
OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1153
Abstract: Abstract MicroRNAs (miRNAs or miRs) are single-stranded, ∼22-nucleotide noncoding RNAs that regulate many cellular processes. While numerous miRNA quantification technologies are available, a recent analysis of 12 commercial platforms revealed high variations in reproducibility, sensitivity,… read more here.
Keywords: mirna quantification; system; quantification; universal hairpin ... See more keywords
Reference Genes for qPCR-Based miRNA Expression Profiling in 14 Human Tissues
Sign Up to like & getrecommendations! Published in 2022 at "Medical Principles and Practice"
DOI: 10.1159/000524283
Abstract: MicroRNAs (miRNAs) are promising biomarkers for the diagnosis and prognosis of various diseases. Quantitative PCR is the most frequently used method of measuring expression levels of miRNA. However, the lack of validated reference genes represents… read more here.
Keywords: expression; genes qpcr; qpcr based; reference genes ... See more keywords
Streptococcus pneumoniae carriage studies in adults: Importance, challenges, and key issues to consider when using quantitative PCR-based approaches
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1122276
Abstract: Streptococcus pneumoniae causes significant morbidity and mortality among older adults. Detection of pneumococcal carriage is an accepted endpoint in pneumococcal conjugate vaccine studies. However, low sensitivity of culture-based approaches and nasopharyngeal samples have hampered adult… read more here.
Keywords: pneumoniae carriage; pneumoniae; based approaches; carriage ... See more keywords
C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Public Health"
DOI: 10.3389/fpubh.2022.787299
Abstract: Background Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide… read more here.
Keywords: macrolide resistance; acne; resistance; qpcr based ... See more keywords