A novel multiplex qPCR method for assessing the comparative lengths of telomeres
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.23929
Abstract: The comparative length of telomeres is considered to be related to diseases such as cancer, aging, and cardiovascular diseases. qPCR is currently one of the main methods for detecting telomere length. However, due to the… read more here.
Keywords: assessing comparative; qpcr method; method assessing; comparative lengths ... See more keywords
Measurement of belowground diversity of fine roots in subtropical forests based on a quantitative real-time PCR (qPCR) method
Sign Up to like & getrecommendations! Published in 2017 at "Plant and Soil"
DOI: 10.1007/s11104-017-3402-y
Abstract: Background and aimsDirect measurement of fine root diversity is very important to unveil belowground interaction, community dynamics and ecosystem functions in forests, but is limited by the effective method. This study attempted to develop specific… read more here.
Keywords: qpcr method; diversity fine; method; fine roots ... See more keywords
A long-amplicon quantitative PCR assay with propidium monoazide to enumerate viable Listeria monocytogenes after heat and desiccation treatments.
Sign Up to like & getrecommendations! Published in 2020 at "Food microbiology"
DOI: 10.1016/j.fm.2019.103310
Abstract: The objective of this study was to develop a qPCR method for specific enumeration of viable Listeria monocytogenes in food processing facilities and heat treated products. Primers specific for L. monocytogenes were designed to amplify… read more here.
Keywords: heat; qpcr; desiccation; qpcr method ... See more keywords
SYBR Green real-time qPCR method: Diagnose drowning more rapidly and accurately.
Sign Up to like & getrecommendations! Published in 2021 at "Forensic science international"
DOI: 10.1016/j.forsciint.2021.110720
Abstract: In the field of drowning research, the method of diatom morphology has been most applied to determine whether the cause of death is drowning. However, the characteristics of complex operation, high level of professional knowledge… read more here.
Keywords: qpcr method; real time; method; sybr green ... See more keywords
Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of medical microbiology"
DOI: 10.1099/jmm.0.001084
Abstract: Introduction. Chlamydia trachomatis (C. trachomatis, CT) is an obligatory intracellular bacterium that causes urogenital tract infections and leads to severe reproductive consequences. Therefore, a rapid and accurate detection method with high sensitivity and specificity is… read more here.
Keywords: qpcr method; method; microbiology; multiplex qpcr ... See more keywords
PMA-qPCR method for the selective quantitation of viable lactic acid bacteria in fermented milk
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.984506
Abstract: The number of viable lactic acid bacteria (LAB) is a key indicator of the quality of fermented milk. Currently, the combination of propidium monoazide (PMA) and qPCR has been applied in the quantification of viable… read more here.
Keywords: fermented milk; pma qpcr; qpcr method; qpcr ... See more keywords