A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes
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DOI: 10.1016/j.btre.2020.e00447
Abstract: Highlights • Quantitative polymerase chain reactions specifically detect Trichoderma atroviride.• The method uses a probe based on the tef-1α for the detection.• The method simultaneously detects T. atroviride and Botrytis cinerea in blackberries. read more here.
Keywords: botrytis cinerea; qpcr taqman; trichoderma atroviride; multiplex qpcr ... See more keywords