Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Biology Reports"
DOI: 10.1007/s11033-021-06153-y
Abstract: Scutellaria baicalensis Georgi is a famous medicinal plant with its dried roots having been used as a traditional Chinese medicinal for more than 2000 years. Although its genome sequence has previously been published and molecular… read more here.
Keywords: scutellaria baicalensis; reference; biology; qrt pcr ... See more keywords
A multiplex sensitive quantification of microRNAs based on competitive PCR
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-016-0364-5
Abstract: MicroRNAs (miRNAs) are endogenous ~22 nt RNAs that play important regulatory roles in animals and plants by targeting mRNAs for cleavage or gene silencing. Although quantitative real-time PCR (qRT-PCR) had been widely used for miRNAs… read more here.
Keywords: mir 505; sensitive quantification; pcr; cpcr ... See more keywords
Detection of phenol contamination in RNA samples and its impact on qRT-PCR results.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.02.002
Abstract: Residual phenol, carried over from RNA purification, can alter RNA concentration measurements and is assumed to inhibit PCR. Here, we demonstrate that Impurities A260 values of spectral content profiling (SCP) UV/Vis measurements correlated with phenol… read more here.
Keywords: rna; phenol contamination; qrt pcr; concentration ... See more keywords
Selection of best reference genes for qRT-PCR analysis of human neural stem cells preconditioned with hypoxia or baicalein-enriched fraction extracted from Oroxylum indicum medicinal plant
Sign Up to like & getrecommendations! Published in 2019 at "Heliyon"
DOI: 10.1016/j.heliyon.2019.e02156
Abstract: Whilst the potential of neural stem cell (NSC)-based treatment is recognized worldwide and seems to offer a promising therapeutic option for stroke treatments, there is currently no full understanding regarding the effects of hypoxic and… read more here.
Keywords: enriched fraction; reference; baicalein enriched; qrt pcr ... See more keywords
Droplet digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of cardiology"
DOI: 10.1016/j.ijcard.2017.10.111
Abstract: BACKGROUND micro-RNAs have shown promise as potential biomarkers for acute myocardial infarction and ischemia-reperfusion injury (I/R). Most recently droplet digital polymerase chain reaction (ddPCR) has been introduced as a more reliable and reproducible method for… read more here.
Keywords: ddpcr; pcr; myocardial infarction; qrt pcr ... See more keywords
Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2021.114382
Abstract: Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria… read more here.
Keywords: sars cov; pcr; mass; qrt pcr ... See more keywords
Discrepancies between feline coronavirus antibody and nucleic acid detection in effusions of cats with suspected feline infectious peritonitis
Sign Up to like & getrecommendations! Published in 2019 at "Research in Veterinary Science"
DOI: 10.1016/j.rvsc.2017.10.004
Abstract: Abstract Intra-vitam diagnosis of feline infectious peritonitis (FIP) is a challenge for veterinary diagnosticians, since there are no highly specific and sensitive assays currently available. With the aim to contribute to fill this diagnostic gap,… read more here.
Keywords: detection; infectious peritonitis; antibody; feline infectious ... See more keywords
Phytoplankton growth characterization in short term MPN culture assays using 18S metabarcoding and qRT-PCR.
Sign Up to like & getrecommendations! Published in 2019 at "Water research"
DOI: 10.1016/j.watres.2019.114941
Abstract: The most probable number dilution-culture assay (MPN) is used to enumerate viable phytoplankton in regulatory tests of ballast water treatment systems. However the United States Coast Guard has not yet accepted MPN, in part due… read more here.
Keywords: qrt pcr; growability; phytoplankton; culture ... See more keywords
Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes
Sign Up to like & getrecommendations! Published in 2021 at "ACS Omega"
DOI: 10.1021/acsomega.0c05635
Abstract: One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one-step qRT-PCR is well suited for the diagnosis of COVID-19 and that there are many… read more here.
Keywords: step; step qrt; one step; qrt pcr ... See more keywords
Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-69423-y
Abstract: Reference genes (RGs) must have a stable expression in tissues in all experimental conditions to normalize real-time quantitative reverse transcription PCR (qRT-PCR) data. F0104 is a highly studied lineage of zebrafish developed to overexpress the… read more here.
Keywords: qrt pcr; evaluation qpcr; stability; reference genes ... See more keywords
Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices.
Sign Up to like & getrecommendations! Published in 2022 at "Nanoscale"
DOI: 10.1039/d2nr02850f
Abstract: The potential for liquid biopsy samples to be used in place of more invasive tissue biopsies has become increasingly revalent as it has been found that nucleic acids (NAs) present in the blood of cancer… read more here.
Keywords: nuclease inhibitors; detergents nuclease; qrt pcr; role detergents ... See more keywords