Inactivation Kinetics of G-Quadruplex/Hemin Complex and Optimization for More Reliable Catalysis
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DOI: 10.1002/cplu.202200090
Abstract: Reliable catalysis is critical for the synthesis of various chemicals, molecular sensing and biomedicine. G-quadruplex/Hemin (GQH) complex, a peroxidase-mimicking DNAzyme, has been widely used in various publications. However, a concern exists about the unstable kinetics… read more here.
Keywords: inactivation kinetics; quadruplex hemin; reliable catalysis;
Effects of Molecular Crowding on G-Quadruplex-hemin Mediated Peroxidase Activity
Sign Up to like & getrecommendations! Published in 2020 at "Chemical Research in Chinese Universities"
DOI: 10.1007/s40242-020-0018-1
Abstract: The concentration of macromolecules in cells can reach up to 50–400 mg/mL. They occupy 40%(volume fraction) of the whole cellar space, known as molecular crowding. The diluted solution condition in vitro is different from the… read more here.
Keywords: condition; peroxidase activity; hemin; molecular crowding ... See more keywords
Highly Sensitive Biosensing Applications of a Magnetically Immobilizable Covalent G-Quadruplex-Hemin DNAzyme Catalytic System.
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DOI: 10.1021/acs.analchem.1c04842
Abstract: G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design of… read more here.
Keywords: system; dnazyme; hemin; quadruplex hemin ... See more keywords
Colorimetric and Photocurrent-Polarity-Switching Photoelectrochemical Dual-Mode Sensing Platform for Highly Selective Detection of Mercury Ions Based on the Split G-Quadruplex-Hemin Complex.
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DOI: 10.1021/acs.analchem.2c03084
Abstract: Mercury ion (Hg2+) is one of the most harmful heavy metal ions with the greatest impact on public health. Herein, based on the excellent catalytic activity toward 3,3',5,5'-tetramethylbenzidine (TMB) and the strong photocurrent-polarity-switching ability to… read more here.
Keywords: split quadruplex; detection; quadruplex hemin; hemin ... See more keywords
Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations.
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DOI: 10.1021/acs.analchem.8b05971
Abstract: Conventional potentiometric ion sensors that rely on a specific ion carrier in a polymeric membrane can hardly achieve multianalyte detection. Inspired by the remarkable ability of built-in logic gate sensors for multianalyte detection, herein we… read more here.
Keywords: dnazyme; based quadruplex; hemin dnazyme; logic gate ... See more keywords
A fluorescent signal-on sensor based on photoinduced electron transfer between copper nanoparticles and G-quadruplex/hemin
Sign Up to like & getrecommendations! Published in 2019 at "Analytical Methods"
DOI: 10.1039/c9ay00461k
Abstract: A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin. read more here.
Keywords: quadruplex hemin; copper; signal; photoinduced electron ... See more keywords
The proximity of the G-quadruplex to hemin impacts the intrinsic DNAzyme activity in mitochondria.
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DOI: 10.1039/d0cc08316j
Abstract: The DNAzyme activity of G-quadruplex/hemin in mitochondria has not been characterized. Herein, we report an unexpected difference in the DNAzyme activity between in vitro assays and in mitochondria. Molecular dynamic simulations illustrate how the interaction… read more here.
Keywords: proximity quadruplex; dnazyme activity; dnazyme; quadruplex hemin ... See more keywords
Zippered G-quadruplex/hemin DNAzyme: exceptional catalyst for universal bioanalytical applications
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DOI: 10.1093/nar/gkab1178
Abstract: Abstract G-quadruplex (G4)/hemin DNAzyme is promising horseradish peroxidase (HRP)-mimic candidate in the biological field. However, its relatively unsatisfactory catalytic capacity limits the potential applications. Inspired by nature protease, we conducted a proximity-enhanced cofactor assembly strategy… read more here.
Keywords: hemin dnazyme; dnazyme exceptional; hemin; quadruplex hemin ... See more keywords
Activatable G-quadruplex based catalases for signal transduction in biosensing
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DOI: 10.1093/nar/gkad031
Abstract: Abstract Discovery of oxidative catalysis with G-quadruplex•hemin constructs prompted a range of exciting developments in the field of biosensor design. Thus, G-quadruplex based DNAzymes with peroxidase activity found a niche as signal transduction modules in… read more here.
Keywords: signal transduction; transduction; quadruplex hemin; based catalases ... See more keywords