Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9674-2_6
Abstract: mRNA turnover plays an important role in the regulation of post-transcriptional gene expression. While many protein factors involved in mRNA degradation have been identified, we still lack a basic understanding of the principles that regulate… read more here.
Keywords: mrna turnover; treat; pipeline; turnover ... See more keywords
Quantification of mRNA cap-modifications by means of LC-QqQ-MS.
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DOI: 10.1016/j.ymeth.2021.05.018
Abstract: Enzymatic modification of the 5'-cap is a versatile approach to modulate the properties of mRNAs. Transfer of methyl groups from S-adenosyl-L-methionine (AdoMet) or functional moieties from non-natural analogs by methyltransferases (MTases) allows for site-specific modifications… read more here.
Keywords: mrna cap; modifications means; cap modifications; quantification mrna ... See more keywords
Quantification of mRNA translation in live cells using single-molecule imaging
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DOI: 10.1038/s41596-019-0284-x
Abstract: mRNA translation is a key step in gene expression. Proper regulation of translation efficiency ensures correct protein expression levels in the cell, which is essential to cell function. Different methods used to study translational control… read more here.
Keywords: microscopy; live cells; mrna translation; translation ... See more keywords