Quantification of the soluble Receptor of Advanced Glycation End-Products (sRAGE) by LC-MS after enrichment by strong cation exchange (SCX) solid-phase extraction (SPE) at the protein level.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.09.050
Abstract: The study of low abundant proteins contributes to increasing our knowledge about (patho)physiological processes and may lead to the identification and clinical application of disease markers. However, studying these proteins is challenging as high-abundant proteins… read more here.
Keywords: cation exchange; enrichment; exchange scx; strong cation ... See more keywords
Affimers as an Alternative to Antibodies in an Affinity LC–MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.8b00414
Abstract: Antibodies are indispensable tools in biomedical research, but their size, complexity, and sometimes lack of reproducibility created a need for the development of alternative binders to overcome these limitations. Affimers are a novel class of… read more here.
Keywords: receptor advanced; method; glycation end; advanced glycation ... See more keywords