A Quantitative PCR Screening Method for Adeno-Associated Viral Vector 2-mediated Gene Doping.
Sign Up to like & getrecommendations! Published in 2021 at "Drug testing and analysis"
DOI: 10.1002/dta.3152
Abstract: Gene therapy is currently prohibited in human and equine athletes and novel analytical methods are needed for its detection. Most in vivo products use non-integrating, recombinant viral vectors derived from adeno-associated virus (AAV) to deliver… read more here.
Keywords: quantitative pcr; gene doping; raav; adeno associated ... See more keywords
Conventional versus real‐time quantitative PCR for rare species detection
Sign Up to like & getrecommendations! Published in 2018 at "Ecology and Evolution"
DOI: 10.1002/ece3.4636
Abstract: Abstract Detection of species in nature at very low abundance requires innovative methods. Conventional PCR (cPCR) and real‐time quantitative PCR (qPCR) are two widely used approaches employed in environmental DNA (eDNA) detection, though lack of… read more here.
Keywords: quantitative pcr; detection; real time; field ... See more keywords
Multiplex and quantitative PCR targeting SCAR markers for strain‐level detection and quantification of biofertilizers
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800318
Abstract: Biofertilizers are the eco‐friendly bio‐input being used to sustain the agriculture by reducing the chemical inputs and improving the soil health. Quality is the major concern of biofertilizer technology which often leads to poor performance… read more here.
Keywords: quantitative pcr; biofertilizer; pcr targeting; pcr ... See more keywords
Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR).
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9833-3_7
Abstract: Assessment of the abundance of fungi in environmental samples by quantitative PCR (qPCR) of community DNA is often a difficult task due to biases introduced during PCR amplification, resulting from the differences associated with length… read more here.
Keywords: quantitative pcr; treatment plants; abundance fungi; pcr qpcr ... See more keywords
Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9833-3_8
Abstract: This chapter reports the use of real-time quantitative PCR to detect Diplodia sapinea, a fungal plant pathogen that causes shoot tip dieback and tree mortality on pine trees. This molecular approach represents a reliable and… read more here.
Keywords: quantitative pcr; fungal plant; time quantitative; real time ... See more keywords
Selection of reference genes for miRNA quantitative PCR and its application in miR-34a/Sirtuin-1 mediated energy metabolism in Megalobrama amblycephala
Sign Up to like & getrecommendations! Published in 2019 at "Fish Physiology and Biochemistry"
DOI: 10.1007/s10695-019-00658-x
Abstract: MiRNAs are small, non-coding RNAs that downregulate gene expression at post-transcriptional levels. They have emerged as important regulators involved in metabolism, immunity, and cancer. Real-time quantitative PCR (RT-qPCR) is an effective and main method for… read more here.
Keywords: quantitative pcr; genes mirna; metabolism; reference ... See more keywords
Analytics of Cerebrospinal Fluid MicroRNA Quantitative PCR Studies
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Neurobiology"
DOI: 10.1007/s12035-018-1422-0
Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate post-transcriptional gene expression. Recent studies have shown that human disease states correlate with measurable differences in the level of circulating miRNAs relative to healthy controls. Thus, there… read more here.
Keywords: quantitative pcr; analytics cerebrospinal; fluid; tlda ... See more keywords
Development of a quantitative PCR assay for detecting Egeria densa in environmental DNA samples
Sign Up to like & getrecommendations! Published in 2020 at "Conservation Genetics Resources"
DOI: 10.1007/s12686-020-01152-w
Abstract: Brazilian elodea (Egeria densa) is an invasive freshwater plant that demonstrates widespread ecological impacts in freshwater ecosystems and causes substantial economic damage. Here, we developed an environmental DNA assay for detection of E. densa to… read more here.
Keywords: quantitative pcr; environmental dna; development quantitative; egeria densa ... See more keywords
A refined DNA methylation detection method using MspJI coupled quantitative PCR.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.06.006
Abstract: DNA methylation is a highly conserved epigenetic modification with critical roles ranging from protection against phage infection in bacteria to the regulation of gene expression in mammals. DNA methylation at specific sequences can be measured… read more here.
Keywords: quantitative pcr; methylation; dna methylation; mspji coupled ... See more keywords
Implication of Pseudo Reference Genes in Normalization of Data from Reverse Transcription-Quantitative PCR.
Sign Up to like & getrecommendations! Published in 2020 at "Gene"
DOI: 10.1016/j.gene.2020.144948
Abstract: Pseudogenes are duplicated or retrotransposed DNA sequences of native functional genes. Amplification of pseudogenes along with gene of interest often produces false positive results, which is an innate problem in Reverse Transcription-quantitative PCR (RT-qPCR). Selecting… read more here.
Keywords: quantitative pcr; transcription quantitative; reference; reverse transcription ... See more keywords
Analysis of ribosomal RNA stability in dead cells of wine yeast by quantitative PCR.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of food microbiology"
DOI: 10.1016/j.ijfoodmicro.2018.01.020
Abstract: During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used… read more here.
Keywords: quantitative pcr; rrna; dead cells; stability ... See more keywords