Leveraging preserved specimens of Nerodia to infer the spatiotemporal dynamics of Ophidiomyces ophidiicola via quantitative polymerase chain reaction
Sign Up to like & getrecommendations! Published in 2023 at "Ecology and Evolution"
DOI: 10.1002/ece3.9998
Abstract: Abstract Ophidiomyces ophidiicola (Oo) is a fungal pathogen and the causative agent of ophidiomycosis that has affected multiple snake taxa across the United States, Europe, and Asia. Ophidiomycosis has often been referred to as an… read more here.
Keywords: polymerase chain; ophidiomyces ophidiicola; quantitative polymerase; chain reaction ... See more keywords
Evaluation of domain of unknown function 1220 (DUF1220) for detection of human genome by quantitative polymerase chain reaction: Potential use in assessing the biodistribution of transplanted therapeutic human cells.
Sign Up to like & getrecommendations! Published in 2020 at "Drug metabolism and pharmacokinetics"
DOI: 10.1016/j.dmpk.2020.11.001
Abstract: The biodistribution profile of cell-based therapy products in animal models is important for evaluation of their safety and efficacy. Because of its quantitative nature and sensitivity, the quantitative polymerase chain reaction (qPCR) is a useful… read more here.
Keywords: biodistribution; duf1220; human genome; quantitative polymerase ... See more keywords
Etiology of Sepsis in Uganda Using a Quantitative Polymerase Chain Reaction-based TaqMan Array Card
Sign Up to like & getrecommendations! Published in 2019 at "Clinical Infectious Diseases"
DOI: 10.1093/cid/ciy472
Abstract: Background Knowledge of causes of sepsis in sub-Saharan Africa is limited. A better understanding of the microbiology of bloodstream infections could improve outcomes. Methods We used a quantitative polymerase chain reaction (qPCR)-based TaqMan Array Card… read more here.
Keywords: sepsis; etiology; chain reaction; blood ... See more keywords
Assessment of quantitative polymerase chain reaction for BCR–ABL1 transcripts in chronic myeloid leukaemia: Are improved outcomes in patients with e14a2 transcripts an artefact of technology?
Sign Up to like & getrecommendations! Published in 2022 at "British Journal of Haematology"
DOI: 10.1111/bjh.18026
Abstract: The clinical outcome of chronic myeloid leukaemia patients has vastly improved since the introduction of tyrosine kinase inhibitor treatment, with a significant proportion of patients able to achieve treatment‐free remission. However, studies have shown that… read more here.
Keywords: chronic myeloid; e14a2 transcripts; polymerase chain; chain reaction ... See more keywords
Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway
Sign Up to like & getrecommendations! Published in 2020 at "BMC Research Notes"
DOI: 10.1186/s13104-020-05191-2
Abstract: This study aimed to test the hypothesis that levobupivacaine has anti-tumour effects on breast cancer cells. Colony formation and transwell assay were used to determine breast cancer cells proliferation. Flow Cytometry (annexin V and PI… read more here.
Keywords: quantitative polymerase; proliferation; breast cancer; cancer cells ... See more keywords
Optimization of multiplex quantitative polymerase chain reaction based on response surface methodology and an artificial neural network-genetic algorithm approach
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0200962
Abstract: Multiplex quantitative polymerase chain reaction (qPCR) has found an increasing range of applications. The construction of a reliable and dynamic mathematical model for multiplex qPCR that analyzes the effects of interactions between variables is therefore… read more here.
Keywords: multiplex qpcr; methodology; qpcr; multiplex quantitative ... See more keywords
Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis
Sign Up to like & getrecommendations! Published in 2017 at "Arquivo Brasileiro De Medicina Veterinaria E Zootecnia"
DOI: 10.1590/1678-4162-9053
Abstract: The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation… read more here.
Keywords: paraffin embedded; healthy skin; chain reaction; quantitative polymerase ... See more keywords
Quantitative polymerase chain reaction in the bioanalytical laboratory and technical and scientific considerations for nonclinical and clinical assay characterization, validation and sample analysis.
Sign Up to like & getrecommendations! Published in 2022 at "Bioanalysis"
DOI: 10.4155/bio-2022-0170
Abstract: In this manuscript, the European Bioanalysis Forum reports back on their discussions on practical and scientific considerations related to bioanalytical applications of quantitative polymerase chain reaction. This publication follows an earlier publication in which the… read more here.
Keywords: chain reaction; polymerase chain; quantitative polymerase; scientific considerations ... See more keywords
Understanding quantitative polymerase chain reaction bioanalysis issues before validation planning: Japan Bioanalysis Forum discussion group.
Sign Up to like & getrecommendations! Published in 2023 at "Bioanalysis"
DOI: 10.4155/bio-2022-0190
Abstract: Investigating the biodistribution of cell and gene therapy products may play an important role in evaluating their safety and pharmacology. As quantitative polymerase chain reaction (qPCR) is often used for these analyses, it is essential… read more here.
Keywords: bioanalysis; chain reaction; polymerase chain; validation ... See more keywords