Quantitative and Real‐Time Evaluation of Human Respiration Signals with a Shape‐Conformal Wireless Sensing System
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203460
Abstract: Respiration signals reflect many underlying health conditions, including cardiopulmonary functions, autonomic disorders and respiratory distress, therefore continuous measurement of respiration is needed in various cases. Unfortunately, there is still a lack of effective portable electronic… read more here.
Keywords: respiration signals; evaluation human; quantitative real; real time ... See more keywords
Selection of stable reference genes for quantitative real-time PCR in the Varroa mite, Varroa destructor.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.21905
Abstract: To investigate the acaricide toxicity and resistance mechanisms in the Varroa mite, it is essential to understand the genetic responses of Varroa mites to acaricides, which are usually evaluated by transcriptional profiling based on quantitative… read more here.
Keywords: reference; varroa mite; quantitative real; expression ... See more keywords
Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7108-4_18
Abstract: Quantitative real-time PCR (qRT-PCR) is a reliable method to determine and monitor microRNA (miRNA) expression profiles in different cells, tissues, and organisms. Although there are several different strategies in performing qRT-PCR to determine miRNA expression,… read more here.
Keywords: real time; quantitative real; time pcr; pcr ... See more keywords
Evaluation of four commercial quantitative real-time PCR kits with inhibited and degraded samples
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-017-1745-9
Abstract: DNA quantification is a vital step in forensic DNA analysis to determine the optimal input amount for DNA typing. A quantitative real-time polymerase chain reaction (qPCR) assay that can predict DNA degradation or inhibitors present… read more here.
Keywords: degraded samples; real time; dna; quantitative real ... See more keywords
Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Virology"
DOI: 10.1007/s00705-018-3921-8
Abstract: We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a… read more here.
Keywords: real time; quantitative real; time; panel multiplex ... See more keywords
Identification of endogenous microRNA references in porcine serum for quantitative real-time PCR normalization
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biology Reports"
DOI: 10.1007/s11033-018-4242-4
Abstract: MicroRNAs (miRNAs) are evolutionarily conserved small non-coding RNAs that regulate the expression of genes, and they affect important biological and physiological states. Circulating miRNAs in blood are useful markers of metabolism and economic traits. Expression… read more here.
Keywords: real time; quantitative real; mir; reference ... See more keywords
Validation of Housekeeping Genes for Gene Expression Analysis in Iwagaki Oyster (Crassostrea nippona) Under Salinity Stress by Quantitative Real-Time PCR
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Ocean University of China"
DOI: 10.1007/s11802-020-4422-4
Abstract: Hypo-salinity can reduce the immunological reaction in Crassostrea nippona , even lead to massive mortality. It is important to understand the molecular mechanism of oyster defense system, while quantitative real-time PCR can be employed in… read more here.
Keywords: real time; quantitative real; time pcr; expression ... See more keywords
Quantitative real-time PCR assay for rapid detection of Pseudomonas amygdali pv. lachrymans in cucumber leaf rinse
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Diseases and Protection"
DOI: 10.1007/s41348-019-00258-0
Abstract: Pseudomonas amygdali pv. lachrymans (Pal) is a bacterium that causes angular leaf spot disease of cucumbers. There is no direct protection against this pathogen, only prevention. Previously published detection systems are labour-intensive and time-consuming. The… read more here.
Keywords: quantitative real; time; real time; amygdali lachrymans ... See more keywords
Selection of reference genes for quantitative real-time PCR analysis in chicken ovary following silver nanoparticle treatment.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and pharmacology"
DOI: 10.1016/j.etap.2017.09.011
Abstract: Reliable results of quantitative real time PCR (qPCR) analysis require normalization of target gene expression level using reference genes (RGs). However housekeeping genes expression may vary under experimental conditions, so selection of the proper RGs… read more here.
Keywords: real time; quantitative real; time pcr; analysis ... See more keywords
Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris
Sign Up to like & getrecommendations! Published in 2021 at "Heliyon"
DOI: 10.1016/j.heliyon.2021.e06253
Abstract: The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized… read more here.
Keywords: real time; quantitative real; reference; caiman latirostris ... See more keywords
A novel quantitative real-time PCR diagnostic assay for fecal and nasal swab detection of an otariid lungworm, Parafilaroides decorus
Sign Up to like & getrecommendations! Published in 2020 at "International Journal for Parasitology: Parasites and Wildlife"
DOI: 10.1016/j.ijppaw.2020.04.012
Abstract: Parafilaroides decorus, also known as sea lion lungworm, is a metastrongyloid nematode that infects otariid hosts, such as the charismatic California sea lion, Zalophus californianus. P. decorus causes bronchointerstitial pneumonia, respiratory distress, reduced ability to… read more here.
Keywords: quantitative real; parafilaroides decorus; pcr; lungworm ... See more keywords