Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7634-8_12
Abstract: We present a method for the quantification of small regulatory RNAs (sRNAs) in bacteria, by combining single-molecule fluorescence in situ hybridization (smFISH), super-resolved single-fluorophore microscopy, and clustering analysis. Compared to smFISH imaging with diffraction-limited fluorescence… read more here.
Keywords: bacterial cells; resolution imaging; imaging small; microscopy ... See more keywords
Quantitative super-resolution imaging of pathological aggregates reveals distinct toxicity profiles in different synucleinopathies
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2205591119
Abstract: Significance We have developed an approach to define the size of small aggregates involved in neurodegenerative disorders at ∼4 nm precision in live cells and in pathological model mouse tissues. We show that aggregates below… read more here.
Keywords: different synucleinopathies; toxicity; super resolution; distinct toxicity ... See more keywords