Accumulation and Pulse Electron Paramagnetic Resonance Spectroscopic Investigation of the 4-Oxidobenzyl Radical Generated in the Radical S-Adenosyl-l-methionine Enzyme HydG.
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DOI: 10.1021/acs.biochem.1c00619
Abstract: The radical S-adenosyl-l-methionine (SAM) enzyme HydG cleaves tyrosine to generate CO and CN- ligands of the [FeFe] hydrogenase H-cluster, accompanied by the formation of a 4-oxidobenzyl radical (4-OB•), which is the precursor to the HydG… read more here.
Keywords: hydg; oxidobenzyl radical; adenosyl methionine; resonance ... See more keywords
Insertion of 4-Demethylwyosine in tRNAPhe Catalyzed by the Radical S-Adenosyl-l-methionine Enzyme TYW1 Entails Oxidative Cleavage of Pyruvate to Form CO2.
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DOI: 10.1021/acs.biochem.2c00519
Abstract: The radical S-adenosyl-l-methionine (SAM) enzyme TYW1 catalyzes the condensation of C-2 and C-3 atoms of pyruvate with N-methylguanosine containing tRNAPhe to form 4-demethylwyosine (imG-14) modified tRNAPhe. The fate of C-1 is not known, and either… read more here.
Keywords: oxidative cleavage; enzyme tyw1; adenosyl methionine; tyw1 ... See more keywords
Leveraging Substrate Promiscuity of a Radical S-Adenosyl-L-methionine RiPP Maturase toward Intramolecular Peptide Cross-Linking Applications
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DOI: 10.1021/acscentsci.2c00501
Abstract: Radical S-adenosyl-l-methionine (RS) enzymes operate on a variety of substrates and catalyze a wide range of complex radical-mediated transformations. Radical non-α-carbon thioether peptides (ranthipeptides) are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs).… read more here.
Keywords: radical adenosyl; cross; cys asp; adenosyl methionine ... See more keywords
Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.
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DOI: 10.1021/jacs.1c12010
Abstract: The biosynthesis of blasticidin S has drawn attention due to the participation of the radical S-adenosyl methionine (SAM) enzyme BlsE. The original assignment of BlsE as a radical-mediated, redox-neutral decarboxylase is unusual because this reaction… read more here.
Keywords: biosynthesis blasticidin; dehydration; enzyme blse; adenosyl methionine ... See more keywords
Reconstitution and Substrate Specificity of the Radical S-Adenosyl-methionine Thiazole C-Methyltransferase in Thiomuracin Biosynthesis.
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DOI: 10.1021/jacs.7b00693
Abstract: Thiomuracin is a thiopeptide antibiotic with potent activity toward Gram-positive drug-resistant bacteria. Thiomuracin is biosynthesized from a precursor peptide, TbtA, by a complex array of posttranslational modifications. One of several intriguing transformations is the C-methylation… read more here.
Keywords: methyltransferase; thiomuracin; radical adenosyl; reconstitution ... See more keywords
A radical S-adenosyl-L-methionine enzyme and a methyltransferase catalyze cyclopropane formation in natural product biosynthesis
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DOI: 10.1038/s41467-018-05217-1
Abstract: Cyclopropanation of unactivated olefinic bonds via addition of a reactive one-carbon species is well developed in synthetic chemistry, whereas natural cyclopropane biosynthesis employing this strategy is very limited. Here, we identify a two-component cyclopropanase system,… read more here.
Keywords: biosynthesis; cyclopropane; methyltransferase; adenosyl methionine ... See more keywords