The utilization of the search engine, Bolt, to decrease search time and increase peptide identifications in hydroxyl radical protein footprinting-based workflows.
Sign Up to like & getrecommendations! Published in 2021 at "Proteomics"
DOI: 10.1002/pmic.202000295
Abstract: Hydroxyl radical protein footprinting (HRPF) utilizes hydroxyl radicals to covalently modify solvent exposed regions of proteins. When coupled with mass spectrometry, HRPF can provide insightful information on protein structural changes including inside cells. However, the… read more here.
Keywords: search engine; search; time; hydroxyl radical ... See more keywords
Structural Investigation of Therapeutic Antibodies Using Hydroxyl Radical Protein Footprinting Methods
Sign Up to like & getrecommendations! Published in 2022 at "Antibodies"
DOI: 10.3390/antib11040071
Abstract: Commercial monoclonal antibodies are growing and important components of modern therapies against a multitude of human diseases. Well-known high-resolution structural methods such as protein crystallography are often used to characterize antibody structures and to determine… read more here.
Keywords: hydroxyl radical; protein footprinting; structural investigation; radical protein ... See more keywords