LAUSR

About LAUSR
Donate
Contact Us

Follow us:

You are not signed in:

Sign Up!


   Articles with "radical sam" as a keyword



Radical SAM enzymes: Nature's choice for radical reactions

Sign Up to like & get
recommendations! Published in 2022 at "FEBS Letters"

DOI: 10.1002/1873-3468.14519

Abstract: Enzymes that use a [4Fe‐4S]1+ cluster plus S‐adenosyl‐l‐methionine (SAM) to initiate radical reactions (radical SAM) form the largest enzyme superfamily, with over half a million members across the tree of life. This review summarizes recent… read more here.

Keywords: radical sam; reactions radical; sam; radical reactions ... See more keywords

Making and breaking carbon-carbon bonds in class C radical SAM methyltransferases.

Sign Up to like & get
recommendations! Published in 2021 at "Journal of inorganic biochemistry"

DOI: 10.1016/j.jinorgbio.2021.111636

Abstract: Radical S-adenosylmethionine (SAM) enzymes utilize a [4Fe-4S]1+ cluster and S-(5'-adenosyl)-L-methionine, (SAM), to generate a highly reactive radical and catalyze what is arguably the most diverse set of chemical reactions for any known enzyme family. At… read more here.

Keywords: sam enzymes; carbon; radical sam; class radical ... See more keywords

Evidence for Porphyrin-Mediated Electron Transfer in the Radical SAM Enzyme HutW

Sign Up to like & get
recommendations! Published in 2023 at "Biochemistry"

DOI: 10.1021/acs.biochem.2c00474

Abstract: Bacteria that infect the human gut must compete for essential nutrients, including iron, under a variety of different metabolic conditions. Several enteric pathogens, including Vibrio cholerae and Escherichia coli O157:H7, have evolved mechanisms to obtain… read more here.

Keywords: radical sam; transfer; electron transfer; electron ... See more keywords

Incorporation of Ni2+, Co2+, and Selenocysteine into the Auxiliary Fe-S Cluster of the Radical SAM Enzyme HydG.

Sign Up to like & get
recommendations! Published in 2019 at "Inorganic chemistry"

DOI: 10.1021/acs.inorgchem.9b01293

Abstract: The radical SAM enzyme HydG generates CO- and CN--containing Fe complexes that are involved in the bioassembly of the [FeFe] hydrogenase active cofactor, the H-cluster. HydG contains a unique 5Fe-4S cluster in which the fifth… read more here.

Keywords: ni2 co2; enzyme hydg; radical sam; sam enzyme ... See more keywords

Hydrogen–Deuterium Exchange Mass Spectrometry Identifies Local and Long-Distance Interactions within the Multicomponent Radical SAM Enzyme, PqqE

Sign Up to like & get
recommendations! Published in 2024 at "ACS Central Science"

DOI: 10.1021/acscentsci.3c01023

Abstract: Interactions among proteins and peptides are essential for many biological activities including the tailoring of peptide substrates to produce natural products. The first step in the production of the bacterial redox cofactor pyrroloquinoline quinone (PQQ)… read more here.

Keywords: sam; radical sam; pqqe; deuterium exchange ... See more keywords

A Prevalent Group of Actinobacterial Radical SAM/SPASM Maturases Involved in Triceptide Biosynthesis.

Sign Up to like & get
recommendations! Published in 2022 at "ACS chemical biology"

DOI: 10.1021/acschembio.2c00621

Abstract: Triceptides are ribosomally synthesized and post-translationally modified peptides characterized by three-residue cyclophanes. The cyclophanes are installed by radical SAM/SPASM maturases referred to as 3-residue cyclophane forming enzymes (3-CyFEs) which catalyze C(sp2)-Cβ(sp3) bond formation on three… read more here.

Keywords: radical sam; spasm; prevalent group; sam spasm ... See more keywords

The radical SAM enzyme HydE generates adenosylated Fe(I) intermediates en route to the [FeFe]-hydrogenase catalytic H-cluster.

Sign Up to like & get
recommendations! Published in 2020 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.0c03802

Abstract: The H-cluster of [FeFe]-hydrogenase consists of a [4Fe-4S]H-subcluster linked by a cysteinyl bridge to a unique organometallic [2Fe]H-subcluster assigned as the site of interconversion between protons and molecular hydrogen. This [2Fe]H-subcluster is assembled by a… read more here.

Keywords: radical sam; fefe hydrogenase; sam enzyme; hyde ... See more keywords

Characterization of a Radical SAM Oxygenase for the Ether Crosslinking in Darobactin Biosynthesis.

Sign Up to like & get
recommendations! Published in 2022 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.2c05565

Abstract: Darobactin A is a ribosomally synthesized, post-translationally modified peptide (RiPP) with potent and broad-spectrum anti-Gram-negative antibiotic activity. The structure of darobactin A is characterized by an ether and C-C crosslinking. However, the specific mechanism of… read more here.

Keywords: radical sam; sam; sam oxygenase; ether crosslinking ... See more keywords

Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis.

Sign Up to like & get
recommendations! Published in 2025 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.5c01006

Abstract: Radical SAM enzymes are the most widespread biocatalysts. These metalloenzymes, using S-adenosyl-l-methionine (SAM) and a [4Fe-4S] cluster as central cofactors, catalyze a broad range of chemically challenging transformations. The vast majority of radical SAM enzymes… read more here.

Keywords: sam; radical sam; biosynthesis; sam enzymes ... See more keywords

Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis.

Sign Up to like & get
recommendations! Published in 2017 at "Nature chemistry"

DOI: 10.1038/nchem.2714

Abstract: Ribosomally synthesized peptides are built out of L-amino acids, whereas D-amino acids are generally the hallmark of non-ribosomal synthetic processes. Here we show that the model bacterium Bacillus subtilis is able to produce a novel… read more here.

Keywords: amino acids; synthesized peptides; radical sam; ribosomally synthesized ... See more keywords

Identification of a radical SAM enzyme involved in the synthesis of archaeosine.

Sign Up to like & get
recommendations! Published in 2019 at "Nature chemical biology"

DOI: 10.1038/s41589-019-0390-7

Abstract: Archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), has been believed to be converted to G+-containing… read more here.

Keywords: q0n trna; radical sam; sam enzyme; sam ... See more keywords