Radical SAM enzymes: Nature's choice for radical reactions
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14519
Abstract: Enzymes that use a [4Fe‐4S]1+ cluster plus S‐adenosyl‐l‐methionine (SAM) to initiate radical reactions (radical SAM) form the largest enzyme superfamily, with over half a million members across the tree of life. This review summarizes recent… read more here.
Keywords: radical sam; reactions radical; sam; radical reactions ... See more keywords
Making and breaking carbon-carbon bonds in class C radical SAM methyltransferases.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of inorganic biochemistry"
DOI: 10.1016/j.jinorgbio.2021.111636
Abstract: Radical S-adenosylmethionine (SAM) enzymes utilize a [4Fe-4S]1+ cluster and S-(5'-adenosyl)-L-methionine, (SAM), to generate a highly reactive radical and catalyze what is arguably the most diverse set of chemical reactions for any known enzyme family. At… read more here.
Keywords: sam enzymes; carbon; radical sam; class radical ... See more keywords
Evidence for Porphyrin-Mediated Electron Transfer in the Radical SAM Enzyme HutW
Sign Up to like & getrecommendations! Published in 2023 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00474
Abstract: Bacteria that infect the human gut must compete for essential nutrients, including iron, under a variety of different metabolic conditions. Several enteric pathogens, including Vibrio cholerae and Escherichia coli O157:H7, have evolved mechanisms to obtain… read more here.
Keywords: radical sam; transfer; electron transfer; electron ... See more keywords
Incorporation of Ni2+, Co2+, and Selenocysteine into the Auxiliary Fe-S Cluster of the Radical SAM Enzyme HydG.
Sign Up to like & getrecommendations! Published in 2019 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.9b01293
Abstract: The radical SAM enzyme HydG generates CO- and CN--containing Fe complexes that are involved in the bioassembly of the [FeFe] hydrogenase active cofactor, the H-cluster. HydG contains a unique 5Fe-4S cluster in which the fifth… read more here.
Keywords: ni2 co2; enzyme hydg; radical sam; sam enzyme ... See more keywords
A Prevalent Group of Actinobacterial Radical SAM/SPASM Maturases Involved in Triceptide Biosynthesis.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00621
Abstract: Triceptides are ribosomally synthesized and post-translationally modified peptides characterized by three-residue cyclophanes. The cyclophanes are installed by radical SAM/SPASM maturases referred to as 3-residue cyclophane forming enzymes (3-CyFEs) which catalyze C(sp2)-Cβ(sp3) bond formation on three… read more here.
Keywords: radical sam; spasm; prevalent group; sam spasm ... See more keywords
The radical SAM enzyme HydE generates adenosylated Fe(I) intermediates en route to the [FeFe]-hydrogenase catalytic H-cluster.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c03802
Abstract: The H-cluster of [FeFe]-hydrogenase consists of a [4Fe-4S]H-subcluster linked by a cysteinyl bridge to a unique organometallic [2Fe]H-subcluster assigned as the site of interconversion between protons and molecular hydrogen. This [2Fe]H-subcluster is assembled by a… read more here.
Keywords: radical sam; fefe hydrogenase; sam enzyme; hyde ... See more keywords
Characterization of a Radical SAM Oxygenase for the Ether Crosslinking in Darobactin Biosynthesis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c05565
Abstract: Darobactin A is a ribosomally synthesized, post-translationally modified peptide (RiPP) with potent and broad-spectrum anti-Gram-negative antibiotic activity. The structure of darobactin A is characterized by an ether and C-C crosslinking. However, the specific mechanism of… read more here.
Keywords: radical sam; sam; sam oxygenase; ether crosslinking ... See more keywords
Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemistry"
DOI: 10.1038/nchem.2714
Abstract: Ribosomally synthesized peptides are built out of L-amino acids, whereas D-amino acids are generally the hallmark of non-ribosomal synthetic processes. Here we show that the model bacterium Bacillus subtilis is able to produce a novel… read more here.
Keywords: amino acids; synthesized peptides; radical sam; ribosomally synthesized ... See more keywords
Identification of a radical SAM enzyme involved in the synthesis of archaeosine.
Sign Up to like & getrecommendations! Published in 2019 at "Nature chemical biology"
DOI: 10.1038/s41589-019-0390-7
Abstract: Archaeosine (G+), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ0)-containing tRNA (q0N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), has been believed to be converted to G+-containing… read more here.
Keywords: q0n trna; radical sam; sam enzyme; sam ... See more keywords
Mechanism of Radical Initiation in the Radical SAM Enzyme Superfamily.
Sign Up to like & getrecommendations! Published in 2023 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-052621-090638
Abstract: Radical S-adenosylmethionine (SAM) enzymes use a site-differentiated [4Fe-4S] cluster and SAM to initiate radical reactions through liberation of the 5'-deoxyadenosyl (5'-dAdo•) radical. They form the largest enzyme superfamily, with more than 700,000 unique sequences currently, and… read more here.
Keywords: radical sam; sam; enzyme superfamily; mechanism radical ... See more keywords