Determining Phosphodiesterase Activity (Radioactive Assay).
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DOI: 10.1007/978-1-4939-7240-1_21
Abstract: Cyclic-di-GMP phosphodiesterases (PDEs) catalyze the hydrolysis of the bacterial second messenger c-di-GMP. This protocol describes a sensitive radioactive assay for PDE activity in which substrate and product can be quickly and easily separated by thin-layer… read more here.
Keywords: radioactive assay; determining phosphodiesterase; assay; activity radioactive ... See more keywords