Loss of protein kinase 2 subunit alpha 2 (CK2α') effect ram sperm function after freezing and thawing process.
Sign Up to like & getrecommendations! Published in 2017 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2017.01.017
Abstract: Protein kinase 2 subunit alpha 2 (CK2α'), a serine/threonine-selective protein kinase, is associated with sperm apoptosis. However, the presence of CK2α' in ram sperm during the freezing-thawing process has not been previously reported. Thus, this… read more here.
Keywords: ck2; protein kinase; ram sperm;
Bovine oviductal fluid (bOF) collected in the follicular or luteal phase of the estrous cycle exerts similar effects on ram sperm kinematics and acrosome reactivity in vitro.
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive biology"
DOI: 10.1016/j.repbio.2019.07.004
Abstract: This study examined the effects of bovine oviductal fluid (bOF) obtained during the follicular or luteal phase of the estrous cycle on ram sperm kinematics, capacitation status and plasma membrane (PM) integrity at various time… read more here.
Keywords: bof; sperm kinematics; kinematics; ram sperm ... See more keywords
Recombinant SPINK3 improves ram sperm quality and in vitro fertility after cryopreservation.
Sign Up to like & getrecommendations! Published in 2019 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2019.12.019
Abstract: Capacitation-like changes affect sperm of several species, such as ram, reducing cell survival and fertilizing competence. Proteins from seminal plasma stabilize sperm plasma membranes, being an interesting focus to develop strategies for improving cryopreserved ram… read more here.
Keywords: quality; spink3; cryopreservation; ram sperm ... See more keywords
Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation.
Sign Up to like & getrecommendations! Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.01.046
Abstract: The aim of this study was to investigate the dynamic changes that ram sperm experience during in vitro capacitation before and after cryopreservation. Using flow cytometry and computer assisted sperm analysis system (CASA), protein tyrosine phosphorylation… read more here.
Keywords: capacitation; vitro capacitation; dynamic changes; cryopreservation ... See more keywords
Effect of mitochondrial uncoupling and glycolysis inhibition on ram sperm functionality
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DOI: 10.1111/rda.12901
Abstract: Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that,… read more here.
Keywords: ram sperm; glycolysis inhibition; sperm functionality; glycolysis ... See more keywords
Post-thaw quality of ram sperm frozen with different concentrations of low-density lipoproteins associated with non-enzymatic antioxidants
Sign Up to like & getrecommendations! Published in 2023 at "Animal Reproduction"
DOI: 10.1590/1984-3143-ar2022-0068
Abstract: Abstract The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with… read more here.
Keywords: quality ram; ram sperm; thaw quality; different concentrations ... See more keywords
Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2021.660165
Abstract: Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol content. Recently,… read more here.
Keywords: desmosterol; cholesterol; ram sperm; sterol ... See more keywords