Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26327
Abstract: During the last decade, directed evolution has become a standard protein engineering strategy to reengineer proteins for industrial applications under high stress conditions (e.g., high temperature, extreme pH, ionic liquids, or organic solvents). The most… read more here.
Keywords: mutagenesis; beneficial positions; random mutagenesis; ceppcr ... See more keywords
A practical random mutagenesis system for Ralstonia solanacearum strains causing bacterial wilt of Pogostemon cablin using Tn5 transposon
Sign Up to like & getrecommendations! Published in 2018 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-018-2581-x
Abstract: A practical random mutagenesis system of Ralstonia solanacearum by electroporation with Tn5 transposon was established, which may be utilized to provide genetic approach to study virulence genes of R. solanacearum strains and create nonpathogenic mutants… read more here.
Keywords: practical random; random mutagenesis; cablin; bacterial wilt ... See more keywords
Enhancement of catalytic performance of a metagenome-derived thermophilic oligosaccharide-specific xylanase by binding module removal and random mutagenesis.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2020.09.008
Abstract: Xylo-oligosaccharide (XO) is a promising pre-biotic with applications in food, feed and healthcare products. XO can be produced by enzymatic digestion of xylan with xylanase. In this study, we aimed to improve the biochemical properties… read more here.
Keywords: x11p; xylanase; removal; random mutagenesis ... See more keywords
Mutagenesis and genome editing in crop improvement: perspectives for the global regulatory landscape.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in plant science"
DOI: 10.1016/j.tplants.2021.08.002
Abstract: Plant breeding depends on broad genetic variation. New allelic variation can be produced by targeted or random mutagenesis. Seemingly, random mutagenesis is outdated because clustered regularly interspaced short palindromic repeats (CRISPR)-Cas technology is much more… read more here.
Keywords: mutagenesis; genome editing; editing crop; random mutagenesis ... See more keywords
Fragment-Directed Random Mutagenesis by the Reverse Kunkel Method.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00086
Abstract: Two fundamentally different approaches are routinely used for protein engineering: user-defined mutagenesis and random mutagenesis, each with its own strengths and weaknesses. Here, we invent a unique mutagenesis protocol, which combines the advantages of user-defined… read more here.
Keywords: kunkel method; mutagenesis; random mutagenesis; method ... See more keywords
Random Mutagenesis Using Error-Prone DNA Polymerases.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097741
Abstract: "Random mutagenesis" is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence. Once developed, these libraries can then be used for several purposes, including structure-function and directed evolution… read more here.
Keywords: mutagenesis; dna; mutagenesis using; random mutagenesis ... See more keywords
PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20163908
Abstract: QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method… read more here.
Keywords: mutagenesis library; quickstep; random mutagenesis; pto quickstep ... See more keywords
Strain Development in Microalgal Biotechnology—Random Mutagenesis Techniques
Sign Up to like & getrecommendations! Published in 2022 at "Life"
DOI: 10.3390/life12070961
Abstract: Microalgal biomass and metabolites can be used as a renewable source of nutrition, pharmaceuticals and energy to maintain or improve the quality of human life. Microalgae’s high volumetric productivity and low impact on the environment… read more here.
Keywords: development microalgal; strain development; microalgal biotechnology; mutagenesis ... See more keywords