Mutational analysis identifies functional Rap1, Su(Hw), and CTCF insulator sites in Arabidopsis thaliana.
Sign Up to like & getrecommendations! Published in 2020 at "Plant cell reports"
DOI: 10.1007/s00299-020-02601-4
Abstract: KEY MESSAGE Genetic analysis identifies multiple, potential protein binding sites important for insulator function in Arabidopsis thaliana: Rap1 site in UASrpg, Su(Hw) site in UASrpg, and CTCF site in BEAD1c. Three non-plant insulators UASrpg, BEAD1c,… read more here.
Keywords: analysis identifies; arabidopsis thaliana; site; rap1 ... See more keywords
Shank2 Binds to aPKC and Controls Tight Junction Formation with Rap1 Signaling during Establishment of Epithelial Cell Polarity.
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DOI: 10.1016/j.celrep.2020.02.088
Abstract: Epithelial cells establish apicobasal polarity by forming tight junctions (TJs) at the apical-lateral boundary, which play fundamental roles in physiological functions. An evolutionarily conserved atypical protein kinase C (aPKC)-partitioning defective (PAR) complex functions as a… read more here.
Keywords: cell polarity; polarity; rap1; shank2 binds ... See more keywords
Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Cell"
DOI: 10.1016/j.molcel.2019.10.025
Abstract: Summary Pioneer transcription factors (pTFs) bind to target sites within compact chromatin, initiating chromatin remodeling and controlling the recruitment of downstream factors. The mechanisms by which pTFs overcome the chromatin barrier are not well understood.… read more here.
Keywords: chromatin; transcription factor; chromatin fiber; rap1 ... See more keywords
Chromosome end protection by RAP1-mediated inhibition of DNA-PK
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DOI: 10.1038/s41586-025-08896-1
Abstract: During classical non-homologous end joining (cNHEJ), DNA-dependent protein kinase (DNA-PK) encapsulates free DNA ends, forming a recruitment platform for downstream end-joining factors including Ligase 4 (LIG4)1. DNA-PK can also bind telomeres and regulate their resection2–4,… read more here.
Keywords: rap1; end; inhibition dna; end joining ... See more keywords
Either Rap1 or Cdc13 can protect telomeric single-stranded 3′ overhangs from degradation in vitro
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DOI: 10.1038/s41598-019-55482-3
Abstract: Telomeres, the DNA-protein structures capping the ends of linear chromosomes, are important for regulating replicative senescence and maintaining genome stability. Telomeres consist of G-rich repetitive sequences that end in a G-rich single-stranded (ss) 3′ overhang,… read more here.
Keywords: single stranded; degradation; either rap1; rap1 ... See more keywords
Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-70111-0
Abstract: Integrin activation is associated with conformational regulation. In this study, we developed a system to evaluate conformational changes in α4β7 integrin. We first inserted the PA tag into the plexin-semaphorin-integrin (PSI) domain of β7 chain,… read more here.
Keywords: integrin regulation; regulation rap1; conformation; rap1 ... See more keywords
Rap1 regulates apical contractility to allow embryonic morphogenesis without tissue disruption and acts in part via Canoe-independent mechanisms
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e22-05-0176
Abstract: Embryonic morphogenesis is powered by dramatic changes in cell shape and arrangement driven by the cytoskeleton and its connections to adherens junctions. This requires robust linkage allowing morphogenesis without disrupting tissue integrity. The small GTPase… read more here.
Keywords: morphogenesis; canoe; morphogenesis without; rap1 ... See more keywords
Trypanosoma brucei RAP1 Has Essential Functional Domains That Are Required for Different Protein Interactions
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DOI: 10.1128/msphere.00027-20
Abstract: Trypanosoma brucei causes human African trypanosomiasis and regularly switches its major surface antigen, VSG, to evade the host immune response. VSGs are expressed from subtelomeres in a monoallelic fashion. TbRAP1, a telomere protein, is essential… read more here.
Keywords: interaction; tbrap1; rap1; functional domains ... See more keywords
Deletion of RAP1 affects iron homeostasis, azole resistance, and virulence in Candida albicans
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DOI: 10.1128/msphere.00155-25
Abstract: ABSTRACT Rap1 is a DNA-binding protein conserved from yeast to mammals for its role in telomeric maintenance. Here, to explore additional functions of Candida albicans Rap1, we performed RNA sequencing analysis. Experimental validations further showed… read more here.
Keywords: rap1; resistance; iron homeostasis; iron ... See more keywords
Differential Use of Rap1 Effectors for Integrin Activation in Platelets and Lymphocytes
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DOI: 10.1182/blood-2020-142720
Abstract: Rap1 is a major convergence point of the platelet and lymphocyte signaling pathways that result in talin1 binding to the integrin β-cytoplasmic domain and consequent integrin activation, effective hemostasis and immune functions. The nature of… read more here.
Keywords: integrin activation; r35e r118e; rap1; activation ... See more keywords
Talin1 is the Principal Platelet Rap1 Effector of Integrin Activation.
Sign Up to like & getrecommendations! Published in 2020 at "Blood"
DOI: 10.1182/blood.2020005348
Abstract: Rap1 is a major convergence point of the platelet signaling pathways that result in talin1 binding to the integrin b cytoplasmic domain and consequent integrin activation, platelet aggregation, and effective hemostasis. The nature of the… read more here.
Keywords: talin1 principal; platelet; rap1; integrin activation ... See more keywords