Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli
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DOI: 10.1002/iub.2162
Abstract: In Escherichia coli, the expression of heterologous genes for the production of recombinant proteins can be challenging due to the codon bias of different organisms. The rare codons AGG and AGA are among the rarest… read more here.
Keywords: agg aga; consecutive rare; production; escherichia coli ... See more keywords
The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species
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DOI: 10.1016/j.btre.2017.01.002
Abstract: Highlights • Triosephosphate isomerases from different species have different numbers of rare codons for E. coli.• They only have rare codons for Arg, which distribute differently in the corresponding sequence.• Protein expression in E. coli… read more here.
Keywords: expression coli; importance arginine; rare codons; different species ... See more keywords
Expression of transgenes enriched in rare codons is enhanced by the MAPK pathway
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DOI: 10.1038/s41598-020-78453-5
Abstract: The ability to translate three nucleotide sequences, or codons, into amino acids to form proteins is conserved across all organisms. All but two amino acids have multiple codons, and the frequency that such synonymous codons… read more here.
Keywords: enriched rare; mapk pathway; expression transgenes; expression ... See more keywords
Widespread position-specific conservation of synonymous rare codons within coding sequences
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DOI: 10.1371/journal.pcbi.1005531
Abstract: Synonymous rare codons are considered to be sub-optimal for gene expression because they are translated more slowly than common codons. Yet surprisingly, many protein coding sequences include large clusters of synonymous rare codons. Rare codons… read more here.
Keywords: within coding; rare codon; rare codons; synonymous rare ... See more keywords
Modification of Transfer RNA Levels Affects Cyclin Aggregation and the Correct Duplication of Yeast Cells
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DOI: 10.3389/fmicb.2020.607693
Abstract: Codon usage bias (the preferential use of certain synonymous codons (optimal) over others is found at the organism level (intergenomic) within specific genomes (intragenomic) and even in certain genes. Whether it is the result of… read more here.
Keywords: lowly expressed; aggregation; codons optimal; expressed proteins ... See more keywords
Distinct responses to rare codons in select Drosophila tissues
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DOI: 10.7554/elife.76893
Abstract: Codon usage bias has long been appreciated to influence protein production. Yet, relatively few studies have analyzed the impacts of codon usage on tissue-specific mRNA and protein expression. Here, we use codon-modified reporters to perform… read more here.
Keywords: codon usage; codon; distinct responses; rare codons ... See more keywords