Investigation of the putative rate‐limiting role of electron transfer in fatty acid desaturation using transfected HEK293T cells
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13622
Abstract: Elevated fatty acid (FA) levels contribute to severe metabolic diseases. Unbalanced oversupply of saturated FAs is particularly damaging, which renders stearoyl‐CoA desaturase (SCD1) activity an important factor of resistance. A SCD1‐related oxidoreductase protects cells against… read more here.
Keywords: hek293t cells; fatty acid; electron transfer; rate limiting ... See more keywords
Protein Design with Fluoroprolines: 4,4‐Difluoroproline Does Not Eliminate the Rate‐Limiting Step of Thioredoxin Folding
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100418
Abstract: C4‐substituted fluoroprolines (4R)‐fluoroproline ((4R)‐Flp) and (4S)‐fluoroproline ((4S)‐Flp) have been used in protein engineering to enhance the thermodynamic stability of peptides and proteins. The electron‐withdrawing effect of fluorine can bias the pucker of the pyrrolidine ring,… read more here.
Keywords: rate limiting; protein design; rate; peptide bond ... See more keywords
Restoration of ligatable "clean" double-strand break ends is the rate-limiting step in the rejoining of ionizing-radiation-induced DNA breakage.
Sign Up to like & getrecommendations! Published in 2020 at "DNA repair"
DOI: 10.1016/j.dnarep.2020.102913
Abstract: Radiotherapy kills malignant cells by generating double-strand breaks (DSBs). Ionizing- radiation (IR) generates "dirty" DSBs, which associates with blocking chemical adducts at DSB ends. Homologous-directed repair (HDR) efficiently removes IR-induced blocking adducts from both 3'… read more here.
Keywords: limiting step; dsbs; rate limiting; restoration ... See more keywords
Mechanism of rate-limiting step switchover for reversible solid oxide cells in H2/H2O atmosphere
Sign Up to like & getrecommendations! Published in 2019 at "Electrochimica Acta"
DOI: 10.1016/j.electacta.2019.135003
Abstract: Abstract Hydrogen-water are the primary reactant-product pair in the fuel electrode of reversible solid oxide cells. A different dependence of polarization resistance on pH2O in fuel cell and electrolysis modes has been proven experimentally, revealing… read more here.
Keywords: mechanism; oxide cells; reversible solid; rate limiting ... See more keywords
Linear Eyring Plots Conceal a Change in the Rate-Limiting Step in an Enzyme Reaction
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b01099
Abstract: The temperature dependence of psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase steady-state rates yields nonlinear and linear Eyring plots, respectively. Solvent viscosity effects and multiple- and single-turnover pre-steady-state kinetics demonstrate that while product release is rate-limiting at… read more here.
Keywords: step; rate limiting; eyring plots; rate ... See more keywords
Accelerating the Low-Temperature Catalytic Oxidation of Acetone over Al-Substituted Mn-Al Oxides by Rate-Limiting Step Modulation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c06186
Abstract: In order to enhance the catalytic activity and improve the stability of Mn-Al oxides in acetone oxidation, it is interesting to have found that modulating and accelerating the rate-limiting step by Al substitution rather than… read more here.
Keywords: limiting step; oxidation; rate limiting; acetone ... See more keywords
Probing the Free Energy Landscape of Organophotoredox-Catalyzed Anti-Markovnikov Hydrofunctionalization of Alkenes.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c07807
Abstract: Experimental 13C kinetic isotope effects (KIEs) provide unprecedented mechanistic insight into three intermolecular anti-Markovnikov alkene hydrofunctionalization reactions─hydroesterification, hydroamination, and hydroetherification─enabled by organophotoredox catalysis. All three reactions are found to proceed via initial oxidation of the… read more here.
Keywords: hydrofunctionalization; anti markovnikov; nucleophilic attack; rate limiting ... See more keywords
CMG helicase can use ATPγS to unwind DNA: Implications for the rate-limiting step in the reaction mechanism
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2119580119
Abstract: Significance The rate-limiting step of hexameric replicative helicases is ill-understood and this study on the eukaryotic replicative helicase CMG identifies that the catalytic ATP hydrolysis step is not the rate-limiting step, implying that a conformational… read more here.
Keywords: cmg; step; dna; rate limiting ... See more keywords
Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Experimental Botany"
DOI: 10.1093/jxb/ery010
Abstract: Autophagy-related proteins Atg5 and Atg7 are rate-limiting components of autophagic flux in Arabidopsis. Overexpression of ATG5 or ATG7 genes stimulates Atg8 lipidation, autophagosome formation, and autophagic flux, leading to improved plant fitness. read more here.
Keywords: components autophagic; limiting components; plant fitness; rate limiting ... See more keywords
The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate release.
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic acids research"
DOI: 10.1093/nar/gkac164
Abstract: The DEAD-box protein Dbp5 is essential for RNA export, which involves regulation by the nucleoporins Gle1 and Nup159 at the cytoplasmic face of the nuclear pore complex (NPC). Mechanistic understanding of how these nucleoporins regulate… read more here.
Keywords: gle1; rate limiting; rate; release ... See more keywords
Transcription closed and open complex formation coordinate expression of genes with a shared promoter region
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the Royal Society Interface"
DOI: 10.1098/rsif.2019.0507
Abstract: Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements,… read more here.
Keywords: transcription; promoter elements; sharing promoter; rate limiting ... See more keywords