Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202400280
Abstract: D‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase). To… read more here.
Keywords: thermostability; semi rational; engineering allulose; allulose epimerase ... See more keywords
Rational engineering of p‐hydroxybenzoate hydroxylase to enable efficient gallic acid synthesis via a novel artificial biosynthetic pathway
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26364
Abstract: Gallic acid (GA) is a naturally occurring phytochemical that has strong antioxidant and antibacterial activities. It is also used as a potential platform chemical for the synthesis of diverse high‐value compounds. Hydrolytic degradation of tannins… read more here.
Keywords: rational engineering; gallic acid; hydroxybenzoate hydroxylase; pathway ... See more keywords
Rational engineering in Escherichia coli for high‐titer production of baicalein based on genome‐scale target identification
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28087
Abstract: Baicalein is a bioactive flavonoid isolated from the traditional Chinese medicinal plant, Scutellaria baicalensis Georgi. Microbial synthesis of flavonoids has been intensively developed owing to the eco‐friendly nature of the process. However, the titer of… read more here.
Keywords: rational engineering; engineering escherichia; escherichia coli; identification ... See more keywords
Functional and Mechanistic Dissection of Protein Glutaminase PG3 and Its Rational Engineering for Enhanced Modification of Myofibrillar Proteins.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c05590
Abstract: Protein glutaminases (PG; EC = 3.5.1.44) are enzymes known for enhancing protein functionality. In this study, we cloned and expressed the gene chryb3 encoding protein glutaminase PG3, exhibiting 39.4 U/mg specific activity. Mature-PG3 featured a… read more here.
Keywords: rational engineering; protein; protein glutaminase; glutaminase pg3 ... See more keywords
Rational engineering of minimally immunogenic nucleases for gene therapy
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DOI: 10.1038/s41467-024-55522-1
Abstract: Genome editing using CRISPR-Cas systems is a promising avenue for the treatment of genetic diseases. However, cellular and humoral immunogenicity of genome editing tools, which originate from bacteria, complicates their clinical use. Here we report… read more here.
Keywords: engineering minimally; genome editing; engineering; rational engineering ... See more keywords
Rational engineering of triple hierarchical pores in carbon nanospheres for superior organic supercapacitive storage
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Materials Chemistry A"
DOI: 10.1039/d5ta03248b
Abstract: Porous carbon stands out as the most promising electrode material for supercapacitors, whereas a well-designed porous architecture is the key to enhancing the supercapacitive performance. This is particularly crucial for... read more here.
Keywords: triple hierarchical; carbon; hierarchical pores; engineering triple ... See more keywords
Toward mechanistic modeling and rational engineering of plant respiration
Sign Up to like & getrecommendations! Published in 2023 at "Plant Physiology"
DOI: 10.1093/plphys/kiad054
Abstract: Abstract Plant respiration not only provides energy to support all cellular processes, including biomass production, but also plays a major role in the global carbon cycle. Therefore, modulation of plant respiration can be used to… read more here.
Keywords: rational engineering; plant respiration; mechanistic modeling; plant ... See more keywords
Rational engineering of adeno‐associated virus capsid enhances human hepatocyte tropism and reduces immunogenicity
Sign Up to like & getrecommendations! Published in 2022 at "Cell Proliferation"
DOI: 10.1111/cpr.13339
Abstract: Gene therapy based on recombinant adeno‐associated viral (rAAV) vectors has been proved to be clinically effective for genetic diseases. However, there are still some limitations, including possible safety concerns for high dose delivery and a… read more here.
Keywords: adeno; rational engineering; associated virus; adeno associated ... See more keywords
Evolutionary tinkering vs. rational engineering in the times of synthetic biology
Sign Up to like & getrecommendations! Published in 2018 at "Life Sciences, Society and Policy"
DOI: 10.1186/s40504-018-0086-x
Abstract: Synthetic biology is not only a contemporary reformulation of the recombinant DNA technologies of the last 30 years, combined with descriptive language imported from electrical and industrial engineering. It is also a new way to interpret… read more here.
Keywords: engineering; rational engineering; synthetic biology; tinkering rational ... See more keywords
Rational Engineering of Chorismate-Related Pathways in Saccharomyces cerevisiae for Improving Tyrosol Production
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2019.00152
Abstract: Tyrosol is extensively used in the pharmaceutical industry as an important natural product from plants. In this study, an exogenous pathway involved in catalyzing tyrosine to tyrosol was introduced into Saccharomyces cerevisiae. Furthermore, The pyruvate… read more here.
Keywords: tyrosol; rational engineering; saccharomyces cerevisiae; production ... See more keywords
Rational Engineering of Phenylalanine Accumulation in Pseudomonas taiwanensis to Enable High-Yield Production of Trans-Cinnamate
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2019.00312
Abstract: Microbial biocatalysis represents a promising alternative for the production of a variety of aromatic chemicals, where microorganisms are engineered to convert a renewable feedstock under mild production conditions into a valuable chemical building block. This… read more here.
Keywords: accumulation; rational engineering; production; taiwanensis ... See more keywords