Optimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) Assays.
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DOI: 10.1101/pdb.prot095018
Abstract: Once primers and probes have been designed and obtained, it is necessary to optimize their concentration for each real-time polymerase chain reaction (PCR) assay. A set of PCRs is assembled in which the concentrations of… read more here.
Keywords: real time; time polymerase; chain reaction; reaction pcr ... See more keywords
Nested Polymerase Chain Reaction (PCR).
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot095182
Abstract: Nested polymerase chain reaction (PCR) is used in situations in which it is necessary to increase the sensitivity and/or specificity of PCR, for example, when amplifying a particular member of a polymorphic gene family or… read more here.
Keywords: reaction pcr; pcr; chain reaction; reaction ... See more keywords