Enriching Cysteine-Containing Peptides Using a Sulfhydryl-Reactive Alkylating Reagent with a Phosphonic Acid Group and Immobilized Metal Affinity Chromatography.
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DOI: 10.1021/acs.jproteome.2c00806
Abstract: The reduction of disulfide bonds and their subsequent alkylation are commonplace in typical proteomics workflows. Here, we highlight a sulfhydryl-reactive alkylating reagent with a phosphonic acid group (iodoacetamido-LC-phosphonic acid, 6C-CysPAT) that facilitates the enrichment of… read more here.
Keywords: phosphonic acid; containing peptides; sulfhydryl reactive; reactive alkylating ... See more keywords