‘Read–through marking’ reveals differential nucleotide composition of read-through and truncated cDNAs in iCLIP
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DOI: 10.12688/wellcomeopenres.14663.1
Abstract: We established a modified iCLIP protocol, called ‘read-through marking’, which facilitates the detection of cDNAs that have not been truncated upon encountering the RNA–peptide complex during reverse transcription (read-through cDNAs). A large proportion of these… read more here.
Keywords: reveals differential; read cdnas; read; read marking ... See more keywords