Identification of Protein Isoforms Using Reference Databases Built from Long and Short Read RNA-Sequencing.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.1c00968
Abstract: Alternative splicing can lead to distinct protein isoforms. These can have different functions in specific cells and tissues or in different developmental stages. In this study, we explored whether transcripts assembled from long read, nanopore-based,… read more here.
Keywords: rna seq; read rna; protein isoforms; identification protein ... See more keywords
Unveiling novel targets of paclitaxel resistance by single molecule long-read RNA sequencing in breast cancer
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-42184-z
Abstract: RNA sequencing has become one of the most common technology to study transcriptomes in cancer, whereas its length limits its application on alternative splicing (AS) events and novel isoforms. Firstly, we applied single molecule long-read… read more here.
Keywords: single molecule; long read; rna; rna sequencing ... See more keywords
PSI-Sigma: a comprehensive splicing-detection method for short-read and long-read RNA-seq analysis
Sign Up to like & getrecommendations! Published in 2019 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz438
Abstract: MOTIVATION Percent Spliced-In (PSI) values are commonly used to report alternative pre-mRNA splicing (AS) changes. Previous PSI-detection tools were limited to specific AS events and were evaluated by in silico RNA-seq data. We developed PSI-Sigma,… read more here.
Keywords: long read; rna; rna seq; read rna ... See more keywords
Transcript capture and ultradeep long-read RNA sequencing (CAPLRseq) to diagnose HNPCC/Lynch syndrome.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of medical genetics"
DOI: 10.1136/jmg-2022-108931
Abstract: PURPOSE Whereas most human genes encode multiple mRNA isoforms with distinct function, clinical workflows for assessing this heterogeneity are not readily available. This is a substantial shortcoming, considering that up to 25% of disease-causing gene… read more here.
Keywords: rna sequencing; rna; read rna; capture ultradeep ... See more keywords
NanoBlot: An R-Package for Visualization of RNA Isoforms from Long Read RNA-sequencing Data.
Sign Up to like & getrecommendations! Published in 2023 at "RNA"
DOI: 10.1261/rna.079505.122
Abstract: RT-PCR and northern blots have long been used to study RNA isoforms usage for single genes. Recently, advancements in long read sequencing have yielded unprecedented information about the usage and abundance of these RNA isoforms.… read more here.
Keywords: sequencing data; rna; read rna; long read ... See more keywords