Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8583-z
Abstract: Culture-independent methods have granted the possibility to study microbial diversity in great detail, but technical issues pose a threat to the accuracy of new findings. Biases introduced during DNA extraction can result in erroneous representations… read more here.
Keywords: sample; extraction; dna extraction; reagent contamination ... See more keywords