Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.07.037
Abstract: Infection with Mycobacterium tuberculosis continues to cause substantial human mortality, in part because of the emergence of antimicrobial resistance. Antimicrobial resistance in tuberculosis is solely the result of chromosomal mutations that modify drug activators or… read more here.
Keywords: resistance; phosphorylation cardiolipin; coprotease; reca ... See more keywords
The Kinetic and Molecular Basis for the Interaction of LexA and Activated RecA Revealed by a Fluorescent Amino Acid Probe.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.9b00886
Abstract: The bacterial DNA damage response (the SOS response) is a key pathway involved in antibiotic evasion and a promising target for combating acquired antibiotic resistance. Activation of the SOS response is controlled by two proteins:… read more here.
Keywords: amino acid; probe; interaction; lexa activated ... See more keywords
ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination
Sign Up to like & getrecommendations! Published in 2017 at "Cell Discovery"
DOI: 10.1038/celldisc.2016.53
Abstract: Homologous recombination (HR), catalyzed in an evolutionarily conserved manner by active RecA/Rad51 nucleofilaments, maintains genomic integrity and promotes biological evolution and diversity. The structures of RecA/Rad51 nucleofilaments provide information critical for the entire HR process.… read more here.
Keywords: reca; homologous recombination; atpase activity;
Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep41081
Abstract: The use of DNA as a structural material for nanometre-scale construction has grown extensively over the last decades. The development of more advanced DNA-based materials would benefit from a modular approach enabling the direct assembly… read more here.
Keywords: dna; strand nicks; assembly; single strand ... See more keywords
Mechanism of the formation of the RecA-ssDNA nucleoprotein filament structure: a coarse-grained approach.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular bioSystems"
DOI: 10.1039/c7mb00486a
Abstract: In prokaryotes, the RecA protein catalyzes the repair and strand exchange of double-stranded DNA. RecA binds to single-stranded DNA (ssDNA) and forms a presynaptic complex in which the protein polymerizes around the ssDNA to form… read more here.
Keywords: nucleoprotein filament; ssdna; filament structure; formation ... See more keywords
Phosphorylation of deinococcal RecA affects its structural and functional dynamics implicated for its roles in radioresistance of Deinococcus radiodurans
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2019.1568916
Abstract: Abstract Deinococcus RecA (DrRecA) protein is a key repair enzyme and contributes to efficient DNA repair of Deinococcus radiodurans. Phosphorylation of DrRecA at Y77 (tyrosine 77) and T318 (threonine 318) residues modifies the structural and… read more here.
Keywords: drreca; deinococcus; deinococcus radiodurans; dna ... See more keywords
Effect of RecA inactivation and detoxification systems on the evolution of ciprofloxacin resistance in Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Antimicrobial Chemotherapy"
DOI: 10.1093/jac/dkab445
Abstract: Abstract Background Suppression of SOS response and overproduction of reactive oxygen species (ROS) through detoxification system suppression enhance the activity of fluoroquinolones. Objectives To evaluate the role of both systems in the evolution of resistance… read more here.
Keywords: reca; resistance; sodb; growth ... See more keywords
E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa442
Abstract: Abstract G-quadruplex (G4) DNA structures can form physical barriers within the genome that must be unwound to ensure cellular genomic integrity. Here, we report unanticipated roles for the Escherichia coli Rep helicase and RecA recombinase… read more here.
Keywords: helicase; rep helicase; stabilizing ligands; reca ... See more keywords
Host cell RecA activates a mobile element-encoded mutagenic DNA polymerase
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac515
Abstract: Abstract Homologs of the mutagenic Escherichia coli DNA polymerase V (pol V) are encoded by numerous pathogens and mobile elements. We have used Rum pol (RumA′2B), from the integrative conjugative element (ICE), R391, as a… read more here.
Keywords: polymerase; rum; rum pol; reca ... See more keywords
A new insight into RecA filament regulation by RecX from the analysis of conformation-specific interactions
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.03.14.484239
Abstract: RecA protein mediates homologous recombination repair in bacteria through assembly of long helical filaments on single-stranded DNA (ssDNA) in an ATP dependent manner. RecX, an important negative regulator of RecA, is known to inhibit RecA… read more here.
Keywords: conformation; reca ssdna; reca; regulation ... See more keywords
Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.1101/2023.01.24.525305
Abstract: Replication fork rescue requires Bacillus subtilis RecA, its negative (SsbA) and positive (RecO) mediators, and fork-processing (RadA/Sms). To understand how they work to promote fork remodeling, reconstituted branched replication intermediates were used. We show that… read more here.
Keywords: nascent lagging; lagging strand; reca; rada sms ... See more keywords