Biochemical and functional characterization of a thermostable RecJ exonuclease from Thermococcus gammatolerans.
Sign Up to like & getrecommendations! Published in 2022 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2022.02.033
Abstract: RecJ is ubiquitous in bacteria and Archaea, and play an important role in DNA replication and repair. Currently, our understanding on biochemical function of archaeal RecJ is incomplete due to limited reports. The genome of… read more here.
Keywords: tga recj; exonuclease; recj; thermococcus gammatolerans ... See more keywords
RecJ 5' Exonuclease Digestion of Oligonucleotide Failure Strands: A "Green" Method of Trityl-On Purification.
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DOI: 10.1021/acs.biochem.7b00010
Abstract: Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, give… read more here.
Keywords: trityl purification; exonuclease; purification; failure strands ... See more keywords
The crystal structure of Pyrococcus furiosus RecJ implicates it as an ancestor of eukaryotic Cdc45.
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DOI: 10.2210/pdb5x4j/pdb
Abstract: RecJ nucleases specifically degrade single-stranded (ss) DNA in the 5' to 3' direction. Archaeal RecJ is different from bacterial RecJ in sequence, domain organization, and substrate specificity. The RecJ from archaea Pyrococcus furiosus (PfuRecJ) also… read more here.
Keywords: crystal structure; eukaryotic cdc45; pyrococcus furiosus; domain ... See more keywords