Metabolic profiling of recombinant Escherichia coli cultivations based on high‐throughput FT‐MIR spectroscopic analysis
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Progress"
DOI: 10.1002/btpr.2378
Abstract: Escherichia coli is one of the most used host microorganism for the production of recombinant products, such as heterologous proteins and plasmids. However, genetic, physiological and environmental factors influence the plasmid replication and cloned gene… read more here.
Keywords: escherichia coli; analysis; recombinant; cell ... See more keywords
Purification of recombinant Aβ(1‐42) and pGlu‐Aβ(3‐42) using preparative SDS‐PAGE
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700154
Abstract: Recombinant expression and purification of amyloid peptides represents a common basis for investigating the molecular mechanisms of amyloid formation and toxicity. However, the isolation of the recombinant peptides is hampered by inefficient separation from contaminants… read more here.
Keywords: sds page; purification; recombinant; preparative sds ... See more keywords
Methods to Synthesize and Assemble Recombinant Keratins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1574-4_10
Abstract: Keratin, as one of the most abundant and underexploited protein sources, is a ubiquitous biological material that commonly exists in epithelial cells. Due to the excellent biocompatibility and biodegradability, keratin is widely used in biomedical… read more here.
Keywords: recombinant; assemble recombinant; synthesize assemble; recombinant keratins ... See more keywords
Vacuolar Targeting and Characterization of Recombinant Antibodies.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7856-4_6
Abstract: Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to… read more here.
Keywords: recombinant; vacuolar targeting; biology; targeting characterization ... See more keywords
Expression and purification of recombinant extracellular sulfatase HSulf-2 allows deciphering of enzyme sub-domain coordinated role for the binding and 6-O-desulfation of heparan sulfate
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03027-2
Abstract: Through their ability to edit 6-O-sulfation pattern of Heparan sulfate (HS) polysaccharides, Sulf extracellular endosulfatases have emerged as critical regulators of many biological processes, including tumor progression. However, study of Sulfs remains extremely intricate and… read more here.
Keywords: expression purification; recombinant; domain; heparan sulfate ... See more keywords
Expression and characterization of rainbow trout Oncorhynchus mykiss recombinant myoglobin.
Sign Up to like & getrecommendations! Published in 2021 at "Fish physiology and biochemistry"
DOI: 10.1007/s10695-021-00991-0
Abstract: Recombinant expression system was established for rainbow trout myoglobin (Mb) considering its unique primary structure of having one unusual deletion and two cysteine residues in contrast to the other fish Mbs. The obtained recombinant Mb… read more here.
Keywords: expression characterization; recombinant; myoglobin; rainbow trout ... See more keywords
Optimization and One-Step Purification of Recombinant V Antigen Production from Yersinia pestis
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biotechnology"
DOI: 10.1007/s12033-019-00234-x
Abstract: The purpose of this study was to develop an efficient and inexpensive method for the useful production of recombinant protein V antigen, an important virulence factor for Yersinia pestis. To this end, the synthetic gene… read more here.
Keywords: step purification; production; recombinant; antigen ... See more keywords
Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2020.107552
Abstract: The manufacturing of recombinant protein is traditionally undertaken in mammalian cell culture. Today, speed, cost and safety are the primary considerations for process improvements in both upstream and downstream manufacturing. Leaders in the biopharmaceutical industry… read more here.
Keywords: cell culture; mammalian cell; culture production; recombinant ... See more keywords
Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo
Sign Up to like & getrecommendations! Published in 2017 at "Developmental and Comparative Immunology"
DOI: 10.1016/j.dci.2017.02.015
Abstract: &NA; Innate immunity is initiated depending on the recognition of certain protein receptors termed pattern recognition receptors (PRRs) of pathogen‐associated molecular patterns (PAMPs) to protect the host from various invading pathogens. As one of the… read more here.
Keywords: rflac; recombinant flagellins; channel catfish; rflaa rflab ... See more keywords
Construction of recombinant Pichia pastoris strains for ammonia reduction by the gdhA and glnA regulatory genes in laying hens.
Sign Up to like & getrecommendations! Published in 2022 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2022.113376
Abstract: Ammonia emissions have become an important environmental challenge for the livestock industry. Probiotics are often used as additives to reduce ammonia, and the ammonia reduction efficiency of common probiotics is approximately 20-40%. In this study,… read more here.
Keywords: pichia pastoris; gdha glna; recombinant;
Functional expression of recombinant hybrid enzymes composed of bacterial and insect's chitinase domains in E. coli.
Sign Up to like & getrecommendations! Published in 2020 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.109492
Abstract: To elucidate the functional alteration of the recombinant hybrid chitinases composed of bacterial and insect's domains, we cloned the constitutional domains from chitinase-encoding cDNAs of a bacterial species, Bacillus thuringiensis (BtChi) and a lepidopteran insect… read more here.
Keywords: composed bacterial; recombinant hybrid; expression recombinant; recombinant ... See more keywords