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   Articles with "recombinant adeno" as a keyword



Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives

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recommendations! Published in 2022 at "Biotechnology Journal"

DOI: 10.1002/biot.202200450

Abstract: Recombinant adeno‐associated virus (rAAV) has established itself as a highly efficacious gene delivery vector with a well characterised safety profile allowing broad clinical application. Recent successes in rAAV‐mediated gene therapy clinical trials will continue to… read more here.

Keywords: small molecule; recombinant adeno; associated virus; adeno associated ... See more keywords

Unveiling Small Non‐Coding RNA Dynamics During Recombinant Adeno‐Associated Virus Production

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recommendations! Published in 2025 at "Biotechnology Journal"

DOI: 10.1002/biot.70092

Abstract: Recombinant adeno‐associated viruses (rAAVs) play a pivotal role in gene therapy, yet the molecular interactions underlying rAAV production in host cells remain incompletely understood. Non‐coding RNAs (ncRNAs), particularly microRNAs (miRNAs) and small nucleolar RNAs (snoRNAs),… read more here.

Keywords: recombinant adeno; adeno associated; non coding; raav ... See more keywords

Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing.

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recommendations! Published in 2023 at "Biotechnology and bioengineering"

DOI: 10.1002/bit.28412

Abstract: Gene therapy is a promising therapeutic approach for genetic and acquired diseases nowadays. Among DNA delivery vectors, recombinant adeno-associated virus (rAAV) is one of the most effective and safest vectors used in commercial drugs and… read more here.

Keywords: raav; recombinant adeno; associated virus; adeno associated ... See more keywords

Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids

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recommendations! Published in 2024 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.28828

Abstract: Recombinant adeno‐associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long‐term transgene expression, broad tropism, and ability to transduce both dividing and nondividing cells. However, rAAV vector production… read more here.

Keywords: recombinant adeno; capsid; transfection; expression ... See more keywords

Transcriptomic features reveal molecular signatures associated with recombinant adeno-associated virus production in HEK293 cells.

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recommendations! Published in 2023 at "Biotechnology progress"

DOI: 10.1002/btpr.3346

Abstract: The development of gene therapies based on recombinant adeno-associated viruses (rAAVs) has grown exponentially, so the current rAAV manufacturing platform needs to be more efficient to satisfy rising demands. Viral production exerts great demand on… read more here.

Keywords: transcriptomic features; recombinant adeno; adeno associated; viral production ... See more keywords

High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions

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recommendations! Published in 2024 at "Biotechnology Progress"

DOI: 10.1002/btpr.3445

Abstract: Recombinant adeno‐associated viral vectors (rAAVs) are one of the most used vehicles for gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve product yield, we optimized the suspension production process of… read more here.

Keywords: multivariate; recombinant adeno; vector; yield ... See more keywords

Prior transient exposure to interleukin-21 delivered by recombinant adeno-associated virus vector protects mice from hepatitis B virus persistence.

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recommendations! Published in 2021 at "Antiviral research"

DOI: 10.1016/j.antiviral.2021.105076

Abstract: Chronic infection of hepatitis B virus (HBV) is a high risk factor for hepatic diseases, such as liver fibrosis, cirrhosis and hepatocellular carcinoma. Non-responders and hyporesponders to HBV vaccine are not protected from HBV infection.… read more here.

Keywords: protects mice; hbv; recombinant adeno; hepatitis virus ... See more keywords

Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

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recommendations! Published in 2017 at "Nature Communications"

DOI: 10.1038/s41467-017-02706-7

Abstract: Recent advances using CRISPR-Cas9 approaches have dramatically enhanced the ease for genetic manipulation in rodents. Notwithstanding, the methods to deliver nucleic acids into pre-implantation embryos have hardly changed since the original description of mouse transgenesis… read more here.

Keywords: embryos; crispr cas9; recombinant adeno; adeno associated ... See more keywords

Strategies to package recombinant Adeno-Associated Virus expressing the N-terminal gasdermin domain for tumor treatment

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recommendations! Published in 2021 at "Nature Communications"

DOI: 10.1038/s41467-021-27407-0

Abstract: Pyroptosis induced by the N-terminal gasdermin domain (GSDMNT) holds great potential for anti-tumor therapy. However, due to the extreme cytoxicity of GSDMNT, it is challenging to efficiently produce and deliver GSDMNT into tumor cells. Here,… read more here.

Keywords: tumor; strategies package; gasdermin domain; terminal gasdermin ... See more keywords
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Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield without contamination

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recommendations! Published in 2022 at "Nature Communications"

DOI: 10.1038/s41467-022-28738-2

Abstract: Recombinant adeno-associated virus (rAAV) shows great promise for gene therapy, however scalability, yield and quality remain significant issues. Here we describe an rAAV manufacturing strategy using a ‘helper’ adenovirus that self-inhibits its major late promoter… read more here.

Keywords: yield; associated virus; adeno associated; production ... See more keywords

Recombinant Adeno-Associated Virus-Mediated Expression of Methamphetamine Antibody Attenuates Methamphetamine-Induced Hyperactivity in Mice

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recommendations! Published in 2017 at "Scientific Reports"

DOI: 10.1038/srep46301

Abstract: Methamphetamine (Meth) is one of the most frequently abused drugs worldwide. Recent studies have indicated that antibodies with high affinity for Meth reduce its pharmacological effects. The purpose of this study was to develop a… read more here.

Keywords: methamphetamine; recombinant adeno; associated virus; expression ... See more keywords