Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200450
Abstract: Recombinant adeno‐associated virus (rAAV) has established itself as a highly efficacious gene delivery vector with a well characterised safety profile allowing broad clinical application. Recent successes in rAAV‐mediated gene therapy clinical trials will continue to… read more here.
Keywords: small molecule; recombinant adeno; associated virus; adeno associated ... See more keywords
Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28412
Abstract: Gene therapy is a promising therapeutic approach for genetic and acquired diseases nowadays. Among DNA delivery vectors, recombinant adeno-associated virus (rAAV) is one of the most effective and safest vectors used in commercial drugs and… read more here.
Keywords: raav; recombinant adeno; associated virus; adeno associated ... See more keywords
Transcriptomic features reveal molecular signatures associated with recombinant adeno-associated virus production in HEK293 cells.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3346
Abstract: The development of gene therapies based on recombinant adeno-associated viruses (rAAVs) has grown exponentially, so the current rAAV manufacturing platform needs to be more efficient to satisfy rising demands. Viral production exerts great demand on… read more here.
Keywords: transcriptomic features; recombinant adeno; adeno associated; viral production ... See more keywords
Prior transient exposure to interleukin-21 delivered by recombinant adeno-associated virus vector protects mice from hepatitis B virus persistence.
Sign Up to like & getrecommendations! Published in 2021 at "Antiviral research"
DOI: 10.1016/j.antiviral.2021.105076
Abstract: Chronic infection of hepatitis B virus (HBV) is a high risk factor for hepatic diseases, such as liver fibrosis, cirrhosis and hepatocellular carcinoma. Non-responders and hyporesponders to HBV vaccine are not protected from HBV infection.… read more here.
Keywords: protects mice; hbv; recombinant adeno; hepatitis virus ... See more keywords
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-02706-7
Abstract: Recent advances using CRISPR-Cas9 approaches have dramatically enhanced the ease for genetic manipulation in rodents. Notwithstanding, the methods to deliver nucleic acids into pre-implantation embryos have hardly changed since the original description of mouse transgenesis… read more here.
Keywords: embryos; crispr cas9; recombinant adeno; adeno associated ... See more keywords
Strategies to package recombinant Adeno-Associated Virus expressing the N-terminal gasdermin domain for tumor treatment
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-27407-0
Abstract: Pyroptosis induced by the N-terminal gasdermin domain (GSDMNT) holds great potential for anti-tumor therapy. However, due to the extreme cytoxicity of GSDMNT, it is challenging to efficiently produce and deliver GSDMNT into tumor cells. Here,… read more here.
Keywords: tumor; strategies package; gasdermin domain; terminal gasdermin ... See more keywords
Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield without contamination
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-28738-2
Abstract: Recombinant adeno-associated virus (rAAV) shows great promise for gene therapy, however scalability, yield and quality remain significant issues. Here we describe an rAAV manufacturing strategy using a ‘helper’ adenovirus that self-inhibits its major late promoter… read more here.
Keywords: yield; associated virus; adeno associated; production ... See more keywords
Recombinant Adeno-Associated Virus-Mediated Expression of Methamphetamine Antibody Attenuates Methamphetamine-Induced Hyperactivity in Mice
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep46301
Abstract: Methamphetamine (Meth) is one of the most frequently abused drugs worldwide. Recent studies have indicated that antibodies with high affinity for Meth reduce its pharmacological effects. The purpose of this study was to develop a… read more here.
Keywords: methamphetamine; recombinant adeno; associated virus; expression ... See more keywords
Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus System
Sign Up to like & getrecommendations! Published in 2017 at "Human Gene Therapy Methods"
DOI: 10.1089/hgtb.2016.151
Abstract: The increase in effective treatments using recombinant adeno-associated viral (rAAV) vectors has underscored the importance of scalable, high-yield manufacturing methods. Previous work from this group reported the use of recombinant herpes simplex virus type 1… read more here.
Keywords: production; system; recombinant adeno; adeno associated ... See more keywords
Manufacturing Clinical Grade Recombinant Adeno-Associated Virus Using Invertebrate Cell Lines.
Sign Up to like & getrecommendations! Published in 2017 at "Human gene therapy"
DOI: 10.1089/hum.2017.042
Abstract: Recombinant adeno-associated virus (rAAV) vectors are proving to be a reliable gene transfer system for several clinical applications, with an increasing body of evidence supporting safety and efficacy. Realizing the clinical and commercial potential of… read more here.
Keywords: invertebrate cell; recombinant adeno; associated virus; raav ... See more keywords
Development and Validation of an Anion-Exchange High-Performance Liquid Chromatography Method for Analysis of Empty Capsids and Capsids Encapsidating Genetic Material in a Purified Preparation of Recombinant Adeno-Associated Virus Serotype 5.
Sign Up to like & getrecommendations! Published in 2021 at "Human gene therapy"
DOI: 10.1089/hum.2020.317
Abstract: The development of various manufacturing platforms and analytical technologies has substantially contributed to successfully translating the recombinant adeno-associated viral vector from the lab to the clinic. The active deployment of these analytical technologies for process… read more here.
Keywords: genetic material; recombinant adeno; method; adeno associated ... See more keywords