Microheterogeneity of Recombinant Antibodies: Analytics and Functional Impact
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Journal"
DOI: 10.1002/biot.201700476
Abstract: Antibodies are typical examples of biopharmaceuticals which are composed of numerous, almost infinite numbers of potential molecular entities called variants or isoforms, which constitute the microheterogeneity of these molecules. These variants are generated during biosynthesis… read more here.
Keywords: antibodies analytics; microheterogeneity recombinant; microheterogeneity; biological properties ... See more keywords
Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Journal"
DOI: 10.1002/biot.202100142
Abstract: Tyrosine sulfation is a post‐translational modification found on many surface receptors and plays an important role in cell‐cell and cell‐matrix interactions. However, tyrosine sulfation of therapeutic antibodies has only been reported very recently. Because of… read more here.
Keywords: tyrosine sulfation; modulating tyrosine; sulfation; recombinant antibodies ... See more keywords
Vacuolar Targeting and Characterization of Recombinant Antibodies.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7856-4_6
Abstract: Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to… read more here.
Keywords: recombinant; vacuolar targeting; biology; targeting characterization ... See more keywords
Recombinant antibodies and their use for food immunoanalysis
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03619-7
Abstract: Antibodies are widely employed as biorecognition elements for the detection of a plethora of compounds including food and environmental contaminants, biomarkers, or illicit drugs. They are also applied in therapeutics for the treatment of several… read more here.
Keywords: recombinant antibodies; food immunoanalysis; antibodies use; food ... See more keywords
Why recombinant antibodies - benefits and applications.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2019.01.012
Abstract: Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological… read more here.
Keywords: antibodies benefits; recombinant antibodies; benefits applications;
Effects of a Multimerized Recombinant Autoantibody Against Amyloid-β
Sign Up to like & getrecommendations! Published in 2021 at "Neuroscience"
DOI: 10.1016/j.neuroscience.2021.03.006
Abstract: Alzheimer's disease (AD) is the most common neurodegenerative disease; thus, the search for a cure or causal therapy has become necessary. Despite intense research on this topic in recent decades, there is no curative therapy… read more here.
Keywords: recombinant autoantibody; multimerized recombinant; autoantibody amyloid; study ... See more keywords
Change-makers bring on recombinant antibodies
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0915-8
Abstract: There’s a growing affinity for recombinant antibodies. Some say it’s also high time for animal-free recombinants. read more here.
Keywords: change makers; bring recombinant; recombinant antibodies; makers bring ... See more keywords
Creation of Recombinant Antibodies: Using 5'-RACE to Amplify Immunoglobulin Sequences.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot093856
Abstract: Modern molecular biology techniques have been applied to the production of therapeutic antibodies. Nonetheless, recombinant antibodies remain the exception rather than the rule for the antibodies used in most research and diagnostic applications. The lack… read more here.
Keywords: creation recombinant; antibodies using; race; gene ... See more keywords
Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biological Engineering"
DOI: 10.1186/s13036-019-0187-y
Abstract: BackgroundThe production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins in mammalian cell lines. However, these… read more here.
Keywords: production; cell lines; chinese hamster; promoter ... See more keywords
Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.856049
Abstract: Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells. However, one of the major bottlenecks restricting… read more here.
Keywords: chinese hamster; hamster ovary; production; recombinant antibodies ... See more keywords
Application of recombinant antibodies for treatment of Clostridioides difficile infection: Current status and future perspective
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.972930
Abstract: Clostridioides difficile (C. difficile), known as the major cause of antibiotic-associated diarrhea, is regarded as one of the most common healthcare-associated bacterial infections worldwide. Due to the emergence of hypervirulent strains, development of new therapeutic… read more here.
Keywords: application; clostridioides difficile; antibodies treatment; difficile infection ... See more keywords