Recombinant Expression and Purification of the Shigella Translocator IpaB.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6649-3_15
Abstract: Type III secretion systems (T3SS) are highly conserved virulence factors employed by a large number of pathogenic gram-negative bacteria. Like many T3SS translocators, recombinant expression of the hydrophobic Shigella protein IpaB requires the presence of… read more here.
Keywords: shigella; expression; ipab; expression purification ... See more keywords
Exploring three different expression systems for recombinant expression of globins: Escherichia coli, Pichia pastoris and Spodoptera frugiperda.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.11.027
Abstract: Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the… read more here.
Keywords: different expression; expression globins; expression; expression systems ... See more keywords
Recombinant expression of nanobodies and nanobody-derived immunoreagents
Sign Up to like & getrecommendations! Published in 2020 at "Protein Expression and Purification"
DOI: 10.1016/j.pep.2020.105645
Abstract: Abstract Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems. When produced as fusions with convenient tags, they become reagents which pair their… read more here.
Keywords: nanobodies nanobody; expression nanobodies; expression; derived immunoreagents ... See more keywords
Recombinant Expression and Chemical Amidation of Isotopically Labeled Native Melittin
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c12631
Abstract: Post-translational modifications are ubiquitous in the eukaryotic proteome. However, these modifications are rarely incorporated in NMR studies of eukaryotic proteins, which are typically produced through recombinant expression in E. coli. Melittin is the primary peptide… read more here.
Keywords: recombinant expression; expression; chemical amidation; native melittin ... See more keywords
Bovine seminal plasma osteopontin: Structural modelling, recombinant expression and its relationship with semen quality.
Sign Up to like & getrecommendations! Published in 2020 at "Andrologia"
DOI: 10.1111/and.13905
Abstract: Osteopontin (OPN) is a multifunctional phosphoprotein that has been linked to fertility in bulls. However, the exact mechanism by which OPN contributes to fertilisation is yet unknown. The biotechnological use of OPN in bovine reproduction… read more here.
Keywords: structural modelling; seminal plasma; recombinant expression; opn ... See more keywords
Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
Sign Up to like & getrecommendations! Published in 2021 at "Microbial Cell Factories"
DOI: 10.1186/s12934-021-01642-y
Abstract: Background The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the clinical stage, such enzymes need to be phased out… read more here.
Keywords: application; recombinant expression; purification; expression barnase ... See more keywords
Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2021.793985
Abstract: Human thymidine phosphorylase (HsTP) is an enzyme with important implications in the field of rare metabolic diseases. Defective mutations of HsTP lead to mitochondrial neurogastrointestinal encephalomyopathy (MNGIE), a disease with a high unmet medical need… read more here.
Keywords: enzyme; human thymidine; engineering; recombinant expression ... See more keywords
Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry
Sign Up to like & getrecommendations! Published in 2022 at "Antioxidants"
DOI: 10.3390/antiox11091731
Abstract: Superoxide dismutase (SOD) is a fundamental antioxidant enzyme that neutralises superoxide ions, one of the main reactive oxygen species (ROS). Extremophile organisms possess enzymes that offer high stability and catalytic performances under a wide range… read more here.
Keywords: cell; food; recombinant expression; expression archaeal ... See more keywords
Recombinant expression in E. coli of human FGFR2 with its transmembrane and extracellular domains
Sign Up to like & getrecommendations! Published in 2017 at "PeerJ"
DOI: 10.7717/peerj.3512
Abstract: Fibroblast growth factor receptors (FGFRs) are a family of receptor tyrosine kinases containing three domains: an extracellular receptor domain, a single transmembrane helix, and an intracellular tyrosine kinase domain. FGFRs are activated by fibroblast growth… read more here.
Keywords: human fgfr2; transmembrane extracellular; transmembrane; recombinant expression ... See more keywords