pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105504
Abstract: Primary recovery of recombinant proteins from E. coli often describes a major challenge in downstream processing. After product release, the target protein usually accounts for only a small amount of total protein and has to… read more here.
Keywords: recovery; recombinant fab; primary recovery; step ... See more keywords
Human Recombinant Fab Fragment Neutralizes Shiga Toxin Type 2 Cytotoxic Effects in vitro and in vivo
Sign Up to like & getrecommendations! Published in 2018 at "Toxins"
DOI: 10.3390/toxins10120508
Abstract: Shiga toxin (Stx) producing Escherichia coli (STEC) is responsible for causing hemolytic uremic syndrome (HUS), a life-threatening thrombotic microangiopathy characterized by thrombocytopenia, hemolytic anemia, and acute renal failure after bacterially induced hemorrhagic diarrhea. Until now,… read more here.
Keywords: cytotoxic effects; recombinant fab; human recombinant; toxin ... See more keywords