Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function
Sign Up to like & getrecommendations! Published in 2021 at "FEBS Letters"
DOI: 10.1002/1873-3468.14072
Abstract: In two proof‐of‐concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked and alternative means for basic characterization of functional eukaryotic… read more here.
Keywords: protein; bgis; protein toxicity; recombinant protein ... See more keywords
In situ detection of protein interactions for recombinant therapeutic enzymes
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27621
Abstract: Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and posttranslational modifications… read more here.
Keywords: protein; protein interactions; recombinant protein; interactions recombinant ... See more keywords
Significant impact of mTORC1 and ATF4 pathways in CHO cell recombinant protein production induced by CDK4/6 inhibitor.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28050
Abstract: The CDK4/6 inhibitor has been shown to increase recombinant protein productivity in Chinese hamster ovary cells (CHO). Therefore, we investigated the mechanism that couples cell cycle inhibitor (CCI) treatment with protein productivity utilizing proteomics and… read more here.
Keywords: recombinant protein; phosphorylation; mtorc1; cdk4 inhibitor ... See more keywords
Selectable high‐yield recombinant protein production in human cells using a GFP/YFP nanobody affinity support
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3409
Abstract: Recombinant protein expression systems that produce high yields of pure proteins and multi‐protein complexes are essential to meet the needs of biologists, biochemists, and structural biologists using X‐ray crystallography and cryo‐electron microscopy. An ideal expression… read more here.
Keywords: protein; yfp nanobody; gfp yfp; expression ... See more keywords
Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris
Sign Up to like & getrecommendations! Published in 2018 at "Yeast"
DOI: 10.1002/yea.3301
Abstract: Pichia pastoris expression system has been widely used in recombinant protein production. So far the majority of heterologous proteins are expressed by methanol inducible promoter PAOX1 and constitutive promoter PGAP. The use of other promoters… read more here.
Keywords: protein production; pichia pastoris; recombinant protein; promoter ... See more keywords
Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_2
Abstract: Despite the importance of recombinant protein production in the academy and industrial fields, many issues concerning the expression of soluble and homogeneous products are still unsolved. Several strategies were developed to overcome these obstacles; however,… read more here.
Keywords: solubility problem; coli; recombinant protein; protein production ... See more keywords
Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7312-5_17
Abstract: High-throughput screening (HTS) systems have emerged as important tools to provide fast and low cost evaluation of several conditions at once since it requires small quantities of material and sample volumes. These characteristics are extremely valuable… read more here.
Keywords: screening hts; high throughput; recombinant protein; purification ... See more keywords
Enhanced Recombinant Protein Production of Soluble, Highly Active and Immobilizable PNGase F
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biotechnology"
DOI: 10.1007/s12033-022-00464-6
Abstract: High resolution analysis of N-glycans can be performed after their endoglycosidase mediated removal from proteins. N-glycosidase F peptide (PNGase F) is one the most frequently used enzyme for this purpose. Because of the significant demand… read more here.
Keywords: pngase; recombinant protein; protein production; production ... See more keywords
Development of a highly efficient production process for recombinant protein expression in Escherichia coli NEB10β
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107612
Abstract: Abstract Recombinant protein expression in E. coli is well described, with multiple strains and process strategies available. However, strains used for cloning and molecular biology purposes are not generally considered for protein expression. Using these… read more here.
Keywords: protein expression; production; process; expression ... See more keywords
Diagnostic accuracy of tests using recombinant protein antigens of Mycobacterium leprae for leprosy: A systematic review.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of infection and public health"
DOI: 10.1016/j.jiph.2019.12.011
Abstract: The aim of this systematic review was to investigate the studies that evaluated the sensitivity and specificity of serologic tests using recombinant protein antigens from Mycobacterium leprae for leprosy diagnosis. We included 13 studies that… read more here.
Keywords: protein; protein antigens; systematic review; using recombinant ... See more keywords
The combination of Brazilian red propolis and recombinant protein rCP01850 in the immunoprophylaxis of Corynebacterium pseudotuberculosis infection in mice.
Sign Up to like & getrecommendations! Published in 2020 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2020.104354
Abstract: The immunomodulatory properties of Brazilian red propolis (BRP) have been already described. Also, propolis have been proved to have antibacterial activity on Corynebacterium pseudotuberculosis. An adjuvant effect of red propolis oil was able to induce… read more here.
Keywords: red propolis; rcp01850; recombinant protein; mice ... See more keywords