Identification of two subtilisin‐like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana
Sign Up to like & getrecommendations! Published in 2020 at "Febs Letters"
DOI: 10.1002/1873-3468.14014
Abstract: The tobacco variant Nicotiana benthamiana has recently emerged as a versatile host for the manufacturing of protein therapeutics, but the fidelity of many recombinant proteins generated in this system is compromised by inadvertent proteolysis. Previous… read more here.
Keywords: serine proteases; subtilisin like; like serine; recombinant proteins ... See more keywords
Ready to use stocks of Agrobacterium tumefaciens can simplify process development for the production of recombinant proteins by transient expression in plants.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201900113
Abstract: The transient expression of recombinant proteins in plant tissues infiltrated with Agrobacterium tumefaciens is an established basic research method that is also widely used for the development and manufacture of plant-made pharmaceuticals (PMPs). However, large-scale… read more here.
Keywords: transient expression; production; ready use; process ... See more keywords
A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000340
Abstract: Incubation at pH 4.0 or blanching at ∼65°C facilitates the purification of biopharmaceutical proteins from plants by precipitating most of the host cell proteins (HCPs) before chromatography. However, both methods are compatible only with pH… read more here.
Keywords: tobacco; temperature; facilitates purification; recombinant proteins ... See more keywords
A multiauxotrophic CHO cell line for the rapid isolation of producers of diverse or high levels of recombinant proteins
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28074
Abstract: Chinese Hamster Ovary (CHO) cells are widely used for the high‐level production of recombinant proteins. We created a multiauxotrophic mutant of CHO‐K1 cells, CHO8A, that is deficient in eight enzymatic steps in the purine/pyrimidine biosynthetic… read more here.
Keywords: cell; line rapid; recombinant proteins; cho cell ... See more keywords
Minimal purification method enables developability assessment of recombinant proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28385
Abstract: Analytical characterization of proteins is a critical task for developing therapeutics and subunit vaccine candidates. Assessing candidates with a battery of biophysical assays can inform the selection of one that exhibits properties consistent with a… read more here.
Keywords: purification; recombinant proteins; purification method; minimal purification ... See more keywords
Repressing expression of difficult-to-express recombinant proteins during the selection process increases productivity of CHO stable pools.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28435
Abstract: More than half of licensed therapeutic recombinant proteins (r-proteins) are manufactured using constitutively-expressing, stably-transfected Chinese hamster ovary (CHO) clones. While constitutive CHO expression systems have proven their efficacy for the manufacturing of monoclonal antibodies, many… read more here.
Keywords: stable pools; recombinant proteins; expression; proteins selection ... See more keywords
A chemoenzymatic approach enables the site‐specific conjugation of recombinant proteins
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201800528
Abstract: Many biotechniques including protein microarray, drug screening, biosensors rely on the immobilization of recombinant proteins on the solid supports. It is well known that random orientation of the immobilized proteins could impair their biologic functions.… read more here.
Keywords: site specific; site; specific conjugation; recombinant proteins ... See more keywords
Establishment of an indirect ELISA method for antibody detection of porcine pseudorabies by recombinant gB, gC, and gD proteins
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28228
Abstract: Pseudorabies virus (PRV), as a neuroherpes virus, leads to heavy economic losses in the pig industry worldwide. This study was designed to establish recombinant PRV glycoprotein B (gB), C, and D proteins as PRV diagnostic… read more here.
Keywords: establishment indirect; indirect elisa; recombinant proteins; elisa method ... See more keywords
Optimizing Chaperone Removal Strategy from Overexpressed Recombinant Proteins : GNE, a Case Study.
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DOI: 10.1007/978-1-0716-1859-2_20
Abstract: In the last two decades, numerous innovative advances, strategies and protocols have been developed and optimized to improve the quality and quantity of soluble recombinant protein production in E. coli. One of the major challenges… read more here.
Keywords: chaperone; removal; protein; chaperone removal ... See more keywords
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.
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DOI: 10.1007/978-1-4939-6412-3_8
Abstract: Protein fusion technology has had a major impact on the efficient production and purification of individual recombinant proteins. The use of genetically engineered affinity and solubility-enhancing polypeptide "tags" has increased greatly in recent years and… read more here.
Keywords: proteins enhance; purification; enhance solubility; solubility ... See more keywords
A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis.
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DOI: 10.1007/978-1-4939-6887-9_21
Abstract: Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and… read more here.
Keywords: expression recombinant; generic protocol; expression; recombinant proteins ... See more keywords