Inserting Cre recombinase into the Prolactin 8a2 gene for decidua‐specific recombination in mice
Sign Up to like & getrecommendations! Published in 2022 at "genesis"
DOI: 10.1002/dvg.23473
Abstract: An estimated 75% of unsuccessful pregnancies are due to implantation failure. Investigating the causes of implantation failure is difficult as decidualization and embryo implantation is a dynamic process. Here, we describe a new decidua‐specific iCre… read more here.
Keywords: decidua specific; icre recombinase; inserting cre; recombinase ... See more keywords
Recombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress.
Sign Up to like & getrecommendations! Published in 2022 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2022.03.017
Abstract: Targeted engineering of mammalian cells has been widely attempted to ensure the efficient production of therapeutic proteins with proper quality during bioprocesses. However, the identification of novel targets for cell engineering is labor-intensive and has… read more here.
Keywords: hyperosmotic stress; resistance hyperosmotic; production; stress ... See more keywords
A Synthetic Recombinase-Based Feedback Loop Results in Robust Expression.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00131
Abstract: Accurate control of a biological process is essential for many critical functions in biology, from the cell cycle to proteome regulation. To achieve this, negative feedback is frequently employed to provide a highly robust and… read more here.
Keywords: synthetic biology; feedback; recombinase; expression ... See more keywords
Identification AAVS1-like locus from the porcine genome and site-specific integration of recombinase-mediated cassette exchange using CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2023 at "Animal biotechnology"
DOI: 10.1080/10495398.2023.2187408
Abstract: Gene integration at site-specific loci is a critical approach for understanding the function of a gene in cells or animals. The AAVS1 locus is a well-known safe harbor for human and mouse studies. In this… read more here.
Keywords: crispr cas9; exchange; rmce; porcine ... See more keywords
Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz585
Abstract: Abstract A common mechanism for inducibly controlling protein function relies on reconstitution of split protein fragments using chemical or light-induced dimerization domains. A protein is split into fragments that are inactive on their own, but… read more here.
Keywords: protein; control; recombinase; split ... See more keywords
A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia
Sign Up to like & getrecommendations! Published in 2019 at "mSphere"
DOI: 10.1128/msphere.00710-19
Abstract: Clostridia are anaerobic bacteria with important roles in intestinal and soil microbiomes. The inability to experimentally modify the genomes of clostridia has limited their study and application in biotechnology. Here, we developed a targetron-recombinase system… read more here.
Keywords: recombinase system; targetron recombinase; clostridia; genome engineering ... See more keywords
Regulation of Recombinase Rag-1 Expression by Female Sex Steroids in Treg and Th17 Lymphocytes: Role of Oncostatin M
Sign Up to like & getrecommendations! Published in 2019 at "Doklady Biochemistry and Biophysics"
DOI: 10.1134/s1607672919010198
Abstract: The effect of estradiol (E2), progesterone (P4), and oncostatin M (OSM) on the differentiation of CD4+ T cells to T regulatory (Treg) lymphocytes and T helpers 17 (Th17) was investigated. The possibility of revision of… read more here.
Keywords: expression; sex steroids; rag expression; recombinase ... See more keywords
Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster.
Sign Up to like & getrecommendations! Published in 2017 at "BioTechniques"
DOI: 10.2144/000114494
Abstract: Rapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a… read more here.
Keywords: melanogaster; drosophila melanogaster; recombinase; phage recombinase ... See more keywords
Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.01696
Abstract: African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security… read more here.
Keywords: swine fever; rpa raa; recombinase; virus ... See more keywords
tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice
Sign Up to like & getrecommendations! Published in 2021 at "eLife"
DOI: 10.7554/elife.66835
Abstract: While Cre-dependent viral systems permit the manipulation of many neuron types, some cell populations cannot be targeted by a single DNA recombinase. Although the combined use of Flp and Cre recombinases can overcome this limitation,… read more here.
Keywords: manipulation; ttargit aavs; aavs mediate; mediate sensitive ... See more keywords