A rapid and highly sensitive multiple detection of human adenovirus type 3, type 7 and respiratory syncytial virus by recombinase‐aided reverse transcription PCR
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24889
Abstract: Polymerase chain reaction (PCR) has been widely used for many pathogen detection. However, PCR technology still suffers from long detection time and insufficient sensitivity. Recombinase‐aided amplification (RAA) is a powerful nucleic acid detection tool with… read more here.
Keywords: recombinase aided; detection; pcr; type ... See more keywords
Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.
Sign Up to like & getrecommendations! Published in 2018 at "Diagnostic microbiology and infectious disease"
DOI: 10.1016/j.diagmicrobio.2017.10.005
Abstract: In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The… read more here.
Keywords: rapid reverse; recombinase aided; transcription recombinase; aided amplification ... See more keywords
Rapid detection of Salmonella with Recombinase Aided Amplification.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.06.011
Abstract: Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice… read more here.
Keywords: recombinase aided; detection; salmonella; aided amplification ... See more keywords
Recombinase-Aided Amplification Combined with Lateral Flow (LF-RAA) Assay for Rapid AAV Genome Detection
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c05660
Abstract: Adeno-associated virus (AAV) is a versatile gene vector that is widely used in mammalian research. In basic studies and large-scale AAV production, genetic testing is ubiquitous and routine polymerase chain reaction (PCR)-based tests limit the… read more here.
Keywords: recombinase aided; aav genome; combined lateral; detection ... See more keywords
Detection of pseudorabies virus with a real-time recombinase-aided amplification assay.
Sign Up to like & getrecommendations! Published in 2021 at "Transboundary and emerging diseases"
DOI: 10.1111/tbed.14241
Abstract: Pseudorabies (PR) is an acute infectious disease of pigs caused by pseudorabies virus (PRV), which has caused great economic losses to the pig industry worldwide. Reliable and timely diagnose is crucial for the surveillance, control… read more here.
Keywords: detection; real time; prv; recombinase aided ... See more keywords
Recombinase-Aided Amplification Assay for Rapid Detection of Hypervirulent Klebsiella pneumoniae (hvKp) and Characterization of the hvKp Pathotype
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.03984-22
Abstract: Klebsiella pneumoniae is the most common opportunistic bacterial species and a major threat to public health. Since the 1990s, hvKp has received increasing attention from public health officials and infectious disease specialists. ABSTRACT Hypervirulent Klebsiella… read more here.
Keywords: recombinase aided; pneumoniae; klebsiella pneumoniae; amplification ... See more keywords
Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2022.922146
Abstract: Background Malaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders. Although these… read more here.
Keywords: using recombinase; recombinase aided; plasmodium; flow ... See more keywords
Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.928025
Abstract: Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification… read more here.
Keywords: psittaci; chlamydia psittaci; recombinase aided; infection ... See more keywords
Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.954657
Abstract: Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method… read more here.
Keywords: recombinase aided; detection method; transcription recombinase; detection ... See more keywords
A Combination of Novel Nucleic Acid Cross-Linking Dye and Recombinase-Aided Amplification for the Rapid Detection of Viable Salmonella in Milk
Sign Up to like & getrecommendations! Published in 2022 at "Foods"
DOI: 10.3390/foods11152375
Abstract: Salmonella, as an important foodborne pathogen, can cause various diseases, such as severe enteritis. In recent years, various types of nucleicacid-intercalating dyes have been utilized to detect viable Salmonella. However, in principle, the performance of… read more here.
Keywords: recombinase aided; viable salmonella; salmonella; milk ... See more keywords
Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay
Sign Up to like & getrecommendations! Published in 2023 at "Life"
DOI: 10.3390/life13020494
Abstract: Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic… read more here.
Keywords: recombinase aided; rapid onsite; detection; aided amplification ... See more keywords