Combining recombinase polymerase amplification and DNA‐templated reaction for SARS‐CoV‐2 sensing with dual fluorescence and lateral flow assay output
Sign Up to like & getrecommendations! Published in 2022 at "Biopolymers"
DOI: 10.1002/bip.23485
Abstract: The early phase of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic was exacerbated by a diagnostic challenge of unprecedented magnitude. In the absence of effective therapeutics or vaccines, breaking the chain of transmission… read more here.
Keywords: templated reaction; dna; reaction; fluorescence ... See more keywords
Rapid Visual Detection of Benzimidazole Resistance In Botrytis cinerea By Recombinase Polymerase Amplification Combined With A Lateral Flow Dipstick.
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DOI: 10.1002/ps.6697
Abstract: BACKGROUND Benzimidazole resistance in B. cinerea is related to the point mutations in the target β-tubulin gene (TUB2). Three mutations (E198A, E198K, E198V) at codon 198 account for most of the resistant strains. A rapid… read more here.
Keywords: polymerase amplification; benzimidazole resistance; recombinase polymerase; resistance ... See more keywords
Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02532-9
Abstract: This study was the first attempt to optimize a recombinase polymerase amplification (RPA) and lateral flow (LF) assay combined with immunomagnetic separation (IMS) for the detection of Vibrio parahaemolyticus in raw oysters. The newly developed… read more here.
Keywords: detection; polymerase amplification; recombinase polymerase; rpa assay ... See more keywords
Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Virology"
DOI: 10.1007/s00705-018-3859-x
Abstract: Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The… read more here.
Keywords: recombinase polymerase; detection; enterovirus; polymerase amplification ... See more keywords
Recombinase polymerase amplification assay for rapid detection of maize chlorotic mottle virus in maize
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Virology"
DOI: 10.1007/s00705-019-04361-3
Abstract: Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA),… read more here.
Keywords: detection; chlorotic mottle; recombinase polymerase; mottle virus ... See more keywords
Recombinase polymerase amplification applied to plant virus detection and potential implications.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.01.021
Abstract: Several isothermal techniques for the detection of plant pathogens have been developed with the advent of molecular techniques. Among them, Recombinase Polymerase Amplification (RPA) is becoming an important technique for the rapid, sensitive and cost-effective… read more here.
Keywords: detection; polymerase amplification; plant; recombinase polymerase ... See more keywords
Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.734987
Abstract: Abstract Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about… read more here.
Keywords: amplification assay; detection; polymerase amplification; enterocytozoon hepatopenaei ... See more keywords
Rapid visualization in the specific detection of Flavobacterium columnare, a causative agent of freshwater columnaris using a novel recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) assay
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DOI: 10.1016/j.aquaculture.2020.735780
Abstract: Abstract Flavobacterium columnare is the causative agent of columnaris, a serious disease affecting numerous freshwater fish species worldwide. Although several molecular protocols have been established to detect this pathogen, there still remains a need to… read more here.
Keywords: detection; flavobacterium columnare; causative agent; columnare ... See more keywords
A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification
Sign Up to like & getrecommendations! Published in 2019 at "Food Control"
DOI: 10.1016/j.foodcont.2019.01.011
Abstract: Abstract Saffron (Crocus sativus), one of the most expensive herbs in China, has been subject to various types of adulteration as a consequence of its high price and limited production. The present work describes a… read more here.
Keywords: saffron; saffron crocus; recombinase polymerase; crocus sativus ... See more keywords
Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.02.006
Abstract: Potato virus Y (PVY) is a global challenge for potato production and the leading cause of seed crop downgrading and rejection for certification. Accurate and timely diagnosis is key to effective control of PVY. Here… read more here.
Keywords: detection; polymerase amplification; potato; recombinase polymerase ... See more keywords
Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2021.114227
Abstract: The rapid detection of novel pathogens including SARS-CoV-2 necessitates the development of easy-to-use diagnostic tests that can be readily adapted and utilized in both clinical laboratories and field settings. Delay in diagnosis has facilitated the… read more here.
Keywords: detection; recombinase polymerase; sars cov; lateral flow ... See more keywords