Comprehensive characterization and simultaneous analysis of overall lipids in reconstructed human epidermis using NPLC/HR-MSn: 1-O-E (EO) Cer, a new ceramide subclass
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-02301-3
Abstract: Stratum corneum lipids are responsible for the skin’s barrier function. They are the final product of epidermis lipid biosynthesis. During this process, lipids evolve from simple to complex structures in three main levels respectively (stratum… read more here.
Keywords: nplc msn; human epidermis; comprehensive characterization; epidermis ... See more keywords
Oligomeric α-Synuclein induces skin degeneration in reconstructed human epidermis
Sign Up to like & getrecommendations! Published in 2022 at "Neurobiology of Aging"
DOI: 10.1016/j.neurobiolaging.2022.02.010
Abstract: Aged and photoaged skin exhibit fine wrinkles that are signs of epidermal inflammation and degeneration. It has been shown that healthy elderly skin expresses amyloidogenic proteins, including α-Synuclein, which are known to oligomerize and trigger… read more here.
Keywords: degeneration; synuclein; degeneration reconstructed; oligomeric synuclein ... See more keywords
Modeling dermatophytosis in reconstructed human epidermis: A new tool to study infection mechanisms and to test antifungal agents
Sign Up to like & getrecommendations! Published in 2017 at "Medical Mycology"
DOI: 10.1093/mmy/myw111
Abstract: &NA; Dermatophytosis is a superficial fungal infection of keratinized structures that exhibits an increasing prevalence in humans and is thus requesting novel prophylactic strategies and therapies. However, precise mechanisms used by dermatophytes to adhere at… read more here.
Keywords: modeling dermatophytosis; fungal infection; infection; human epidermis ... See more keywords
Particulate matter 2.5 induced hyperpigmentation in reconstructed human epidermis model (MelaKutis®)
Sign Up to like & getrecommendations! Published in 2022 at "Chinese Medical Journal"
DOI: 10.1097/cm9.0000000000001934
Abstract: To the Editor: Epidemiological studies have confirmed Biocell Biotechnology), and NHEMs in Medium 254 that fine particulate matter (PM2.5) is related to skin hyperpigmentation, but experimental evidence is lacking. Here, we used normal human epidermal… read more here.
Keywords: reconstructed human; particulate matter; human epidermis; hyperpigmentation ... See more keywords
Reproducing the scalp microbiota community: co‐colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Cosmetic Science"
DOI: 10.1111/ics.12688
Abstract: A 3D reconstructed human epidermis (RHE) model colonized with specific microbial strains was developed to model the complex interactions between strains of the human scalp hair. read more here.
Keywords: reproducing scalp; microbiota community; human epidermis; scalp microbiota ... See more keywords
Application of an in vitro reconstructed human skin on cosmetics in skin irritation tests
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Cosmetic Dermatology"
DOI: 10.1111/jocd.13789
Abstract: At present, it is no longer possible to use animal testing for ingredients and cosmetic products in the Organisation for Economic Co‐operation and Development (OECD) member states. However, in vitro tests are widely used to… read more here.
Keywords: human skin; skin cosmetics; application vitro; cosmetics skin ... See more keywords
Stable reconstructed human gingiva–microbe interaction model: Differential response to commensals and pathogens
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2022.991128
Abstract: Background To investigate human oral health and disease, models are required which represent the interactions between the oral mucosa and microbiome. Our aim was to develop an organotypic model which maintains viability of both host… read more here.
Keywords: rhg; reconstructed human; human gingiva; model ... See more keywords
Improved Tool for Predicting Skin Irritation on Reconstructed Human Epidermis Models Based on Electrochemical Impedance Spectroscopy
Sign Up to like & getrecommendations! Published in 2023 at "Biosensors"
DOI: 10.3390/bios13020162
Abstract: The rabbit skin irritation test has been the standard for evaluating the irritation potential of chemicals; however, alternative methods that do not use animal testing are actively encouraged. Reconstructed human epidermis (RhE) models mimic the… read more here.
Keywords: spectroscopy; reconstructed human; electrochemical impedance; irritation ... See more keywords
Treatment with Docosahexaenoic Acid Improves Epidermal Keratinocyte Differentiation and Ameliorates Inflammation in Human Keratinocytes and Reconstructed Human Epidermis Models
Sign Up to like & getrecommendations! Published in 2019 at "Molecules"
DOI: 10.3390/molecules24173156
Abstract: Atopic dermatitis (AD) is a chronic inflammatory skin disease that can cause skin barrier function damage. Although co-incubation with docosahexaenoic acid (DHA) exerts a positive effect on deficient skin models, no studies have investigated the… read more here.
Keywords: epidermal keratinocyte; docosahexaenoic acid; treatment; human epidermis ... See more keywords
Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation
Sign Up to like & getrecommendations! Published in 2020 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics12111041
Abstract: The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages. RHEs remain costly, however,… read more here.
Keywords: rhe; permeation; barrier function; human epidermis ... See more keywords
Proof-of-Concept Organ-on-Chip Study: Topical Cinnamaldehyde Exposure of Reconstructed Human Skin with Integrated Neopapillae Cultured under Dynamic Flow
Sign Up to like & getrecommendations! Published in 2022 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics14081529
Abstract: Pharmaceutical and personal care industries require human representative models for testing to ensure the safety of their products. A major route of penetration into our body after substance exposure is via the skin. Our aim… read more here.
Keywords: proof concept; skin; reconstructed human; human skin ... See more keywords