The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/7651_2020_322
Abstract: This chapter describes the use of fluorescence recovery after photobleaching (FRAP) for analyzing gap junctional intercellular communication (GJIC) among osteocytes in chick calvariae by confocal laser scanning microscope. read more here.
Keywords: junctional intercellular; recovery photobleaching; fluorescence recovery; among osteocytes ... See more keywords
Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching.
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical journal"
DOI: 10.1016/j.bpj.2019.08.030
Abstract: Cells contain numerous membraneless organelles that assemble by intracellular liquid-liquid phase separation. The viscous properties and associated biomolecular mobility within these condensed phase droplets, or condensates, are increasingly recognized as important for cellular function and… read more here.
Keywords: phase; dynamics phase; quantifying dynamics; recovery photobleaching ... See more keywords
Plasma Membrane Protein Fluorescence Recovery after Photobleaching Measured by Confocal Microscope with High Numerical Aperture at Periphery of Live Cell.
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927618007389
Abstract: Since its development in 1970s, fluorescence recovery after photobleaching (FRAP) became widely used method for measuring of protein mobility in live cells. The measurement starts by imaging of the initial distribution of the fluorescently-tagged protein… read more here.
Keywords: protein; recovery; fluorescence; microscope ... See more keywords
DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Microscopy"
DOI: 10.1111/jmi.12989
Abstract: Conventional analysis of fluorescence recovery after photobleaching (FRAP) data for diffusion coefficient estimation typically involves fitting an analytical or numerical FRAP model to the recovery curve data using non‐linear least squares. Depending on the model,… read more here.
Keywords: recovery; deep neural; analysis; recovery photobleaching ... See more keywords
Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1010573
Abstract: Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood. However, the classical… read more here.
Keywords: recovery photobleaching; fluorescence recovery; profile curves; analysis ... See more keywords
Determining vitreous viscosity using fluorescence recovery after photobleaching
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0261925
Abstract: Purpose Vitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules. The central pig vitreous is… read more here.
Keywords: diffusion; recovery photobleaching; fluorescence recovery; vitreous viscosity ... See more keywords