Off-target effects of base editors: what we know and how we can reduce it.
Sign Up to like & getrecommendations! Published in 2021 at "Current genetics"
DOI: 10.1007/s00294-021-01211-1
Abstract: The recently discovered CRISPR-Cas9 modification, base editors (BEs), is considered as one of the most promising tools for correcting disease-causing mutations in humans, since it allows point substitutions to be edited without generating double-stranded DNA… read more here.
Keywords: target effects; effects base; genetics; base editors ... See more keywords
ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac183
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology has been widely used to facilitate efficient genome editing. Current popular sgRNA design tools only consider the sgRNA perfectly matched to the target site and… read more here.
Keywords: exsgrna reduce; target; reduce target; efficiency target ... See more keywords
siRNA Specificity: RNAi Mechanisms and Strategies to Reduce Off-Target Effects
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2020.526455
Abstract: Short interfering RNAs (siRNAs) are processed from long double-stranded RNA (dsRNA), and a guide strand is selected and incorporated into the RNA-induced silencing complex (RISC). Within RISC, a member of the Argonaute protein family directly… read more here.
Keywords: target effects; sirna specificity; specificity rnai; rnai mechanisms ... See more keywords