Engineering Carboxylic Acid Reductase (CAR) through A Whole-Cell Growth-Coupled NADPH Recycling Strategy.
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DOI: 10.1021/acssynbio.0c00290
Abstract: Rapid evolution of enzyme activities is often hindered by the lack of efficient and affordable methods to identify beneficial mutants. We report the development of a new growth-coupled selection method for evolving NADPH-consuming enzymes based… read more here.
Keywords: reductase car; whole cell; growth coupled; acid reductase ... See more keywords