Development and application of a rapid, user-friendly, and inexpensive method to detect Dehalococcoides sp. reductive dehalogenase genes from groundwater
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8203-y
Abstract: TaqMan probe-based quantitative polymerase chain reaction (qPCR) specific to the biomarker reductive dehalogenase (RDase) genes is a widely accepted molecular biological tool (MBT) for determining the abundance of Dehalococcoides sp. in groundwater samples from chlorinated… read more here.
Keywords: groundwater; reductive dehalogenase; development application; method ... See more keywords
Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-019-05571-7
Abstract: Polychlorinated biphenyls (PCBs) contaminate 19% of US Superfund sites and represent a serious risk to human and environmental health. One promising strategy to remediate PCB-contaminated sediments utilizes organohalide-respiring bacteria (OHRB) that dechlorinate PCBs.However, functional genes… read more here.
Keywords: pcb dechlorination; reductive dehalogenase; sediment microcosms; dehalogenase genes ... See more keywords
Heterologous Production and Purification of a Functional Chloroform Reductive Dehalogenase.
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DOI: 10.1021/acschembio.7b00846
Abstract: Reductive dehalogenases (RDases) are key enzymes involved in the respiratory process of anaerobic organohalide respiring bacteria (ORB). Heterologous expression of respiratory RDases is desirable for structural and functional studies; however, there are few reports of… read more here.
Keywords: heterologous production; reductive dehalogenase; expression; chloroform ... See more keywords
Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase Operon pceABCT
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.838026
Abstract: Organohalide respiration (OHR) is a bacterial anaerobic process that uses halogenated compounds, e.g., tetrachloroethene (PCE), as terminal electron acceptors. Our model organisms are Dehalobacter restrictus strain PER-K23, an obligate OHR bacterium (OHRB), and Desulfitobacterium hafniense… read more here.
Keywords: stoichiometry gene; reductive dehalogenase; stoichiometry; gene products ... See more keywords